Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Slfn5 Double Nickase Plasmid (h): sc-408333-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Slfn5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Slfn5 Double Nickase Plasmid (h) and Slfn5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLFN5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Slfn5 Antibody (4G2): sc-293255
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Slfn5 Double Nickase Plasmid (h) | sc-408333-NIC | 20 µg | $410.00 | |||
Slfn5 Double Nickase Plasmid (h2) | sc-408333-NIC-2 | 20 µg | $410.00 |
Human SLFN5 (Slfn5) is an interferon-stimulated Schlafen family protein implicated in regulating transcriptional programs linked to innate immunity, cell-cycle control, and cellular differentiation. Slfn5 has been reported to modulate gene expression downstream of interferon/JAK–STAT signaling and to influence inflammatory and antiviral response networks, including effects on chromatin-associated transcriptional regulation. Altered SLFN5 expression has been associated with changes in tumor cell proliferation, migration, and immune-related gene signatures across multiple cancer contexts, supporting its utility as a mechanistic node in studies of oncogenic and inflammatory signaling. These properties make SLFN5 a relevant target for dissecting interferon-driven pathways, transcriptional regulation, and context-dependent phenotypes in human cell models.
Slfn5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLFN5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLFN5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLFN5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLFN5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.