Slfn14 Double Nickase Plasmid (h): sc-415573-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Slfn14 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Slfn14 Double Nickase Plasmid (h) and Slfn14 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLFN14. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Slfn14 Double Nickase Plasmid (h) | sc-415573-NIC | 20 µg | $410.00 | |||
Slfn14 Double Nickase Plasmid (h2) | sc-415573-NIC-2 | 20 µg | $410.00 |
SLFN14 (Schlafen family member 14) encodes Slfn14, a putative RNA-associated protein implicated in regulation of translation and RNA metabolism during cellular stress and differentiation. As part of the Schlafen gene family, Slfn14 has been linked to modulation of immune-related programs and control of protein synthesis, with effects that intersect pathways governing ribosome function and post-transcriptional regulation. In humans, variants in SLFN14 are associated with inherited thrombocytopenia and platelet function defects, supporting a role in megakaryocyte maturation and platelet biology. These features make SLFN14 a useful target for dissecting mechanisms of hematopoiesis, RNA handling, and gene-expression control in relevant cell models.
Slfn14 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLFN14 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLFN14. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLFN14 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLFN14-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.