Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Slfn11 Double Nickase Plasmid (h): sc-401137-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Slfn11 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Slfn11 Double Nickase Plasmid (h) and Slfn11 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLFN11. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Slfn11 Antibody (E-4): sc-374339
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Slfn11 Double Nickase Plasmid (h) | sc-401137-NIC | 20 µg | $410.00 | |||
Slfn11 Double Nickase Plasmid (h2) | sc-401137-NIC-2 | 20 µg | $410.00 |
SLFN11 (Slfn11) is a nuclear DNA/RNA-associated factor that modulates cellular responses to replication stress and genotoxic damage, shaping genome stability and cell-cycle progression. It is linked to DNA damage response signaling and replication fork dynamics, where it can influence the outcome of stalled replication and transcription–replication conflicts. SLFN11 activity intersects with pathways engaged by topoisomerase inhibition and DNA crosslink repair processes, making it a widely studied determinant of stress tolerance in proliferating cells. Dysregulation of SLFN11 expression or function has been explored in cancer biology and other conditions where altered DNA repair capacity and replication stress are prominent.
Slfn11 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLFN11 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLFN11. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLFN11 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLFN11-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.