SLC6A15 CRISPR/Cas9 KO Plasmid (h): sc-410592
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLC6A15 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SLC6A15 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLC6A15 CRISPR/Cas9 KO Plasmid (h) | sc-410592 | 20 µg | $397.00 |
Overview
SLC6A15 encodes a sodium-dependent neutral amino acid transporter of the SLC6 family that mediates cellular uptake of branched-chain and other neutral amino acids, contributing to amino acid homeostasis and neurotransmitter-related metabolism. It is enriched in neural tissues and supports processes such as nutrient sensing, synaptic function, and metabolic coupling through pathways linked to amino acid availability and cellular stress responses. Variation or altered expression of SLC6A15 has been associated in research studies with neuropsychiatric phenotypes and stress-related biology, reflecting its role in neuronal metabolic resilience. As a membrane transporter, SLC6A15 also provides a tractable entry point for studying transporter regulation, trafficking, and substrate-dependent signaling in human cell models.
SLC6A15 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC6A15 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC6A15 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC6A15 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SLC6A15 protein expression.
This CRISPR knockout system enables efficient generation of SLC6A15-deficient cell models for investigation of SLC6A15 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC6A15 exon(s) critical for SLC6A15 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC6A15 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SLC6A15 CRISPR/Cas9 KO Plasmid (h) and SLC6A15 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC6A15 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SLC6A15 HDR Plasmid (h) and SLC6A15 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC6A15 homology arms to support homology-directed repair at defined SLC6A15 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.