SLC6A14 Double Nickase Plasmid (h): sc-408198-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLC6A14 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SLC6A14 Double Nickase Plasmid (h) and SLC6A14 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC6A14. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLC6A14 Double Nickase Plasmid (h) | sc-408198-NIC | 20 µg | $410.00 | |||
SLC6A14 Double Nickase Plasmid (h2) | sc-408198-NIC-2 | 20 µg | $410.00 |
SLC6A14 (ATB0,+) encodes a Na+/Cl−-dependent plasma membrane transporter that mediates broad-spectrum uptake of neutral and cationic amino acids, including branched-chain and essential amino acids. By regulating intracellular amino acid availability, SLC6A14 influences nutrient-sensing and metabolic signaling pathways such as mTORC1, supports protein synthesis, and shapes redox balance through amino acid flux. Expression and activity of SLC6A14 have been linked to epithelial transport programs and altered nutrient acquisition in several disease contexts, making it a useful target for studying metabolic reprogramming and transporter biology. Functional interrogation of SLC6A14 also informs mechanisms of amino acid-dependent growth control, stress adaptation, and membrane transport regulation in human cells.
SLC6A14 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC6A14 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC6A14. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC6A14 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC6A14-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.