SLC6A14 CRISPR/Cas9 KO Plasmid (h): sc-408198
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLC6A14 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SLC6A14 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLC6A14 CRISPR/Cas9 KO Plasmid (h) | sc-408198 | 20 µg | $397.00 |
Overview
SLC6A14 (also known as ATB0,+) encodes a sodium- and chloride-dependent plasma membrane transporter that mediates concentrative uptake of neutral and cationic amino acids, with notable capacity for broad-spectrum substrates including essential amino acids. By regulating intracellular amino acid availability, SLC6A14 influences nutrient-sensing and metabolic signaling pathways such as mTORC1, shaping cellular growth, redox balance, and stress adaptation. Its expression has been linked to epithelial transport physiology and altered amino acid flux in disease contexts where nutrient acquisition and metabolism are rewired. As a solute carrier at the cell surface, SLC6A14 provides a tractable node for dissecting amino acid transport contributions to proliferation, differentiation, and immune-metabolic interactions.
SLC6A14 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC6A14 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC6A14 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC6A14 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SLC6A14 protein expression.
This CRISPR knockout system enables efficient generation of SLC6A14-deficient cell models for investigation of SLC6A14 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC6A14 exon(s) critical for SLC6A14 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC6A14 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SLC6A14 CRISPR/Cas9 KO Plasmid (h) and SLC6A14 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC6A14 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SLC6A14 HDR Plasmid (h) and SLC6A14 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC6A14 homology arms to support homology-directed repair at defined SLC6A14 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.