SLC35C2 Double Nickase Plasmid (h): sc-409264-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLC35C2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SLC35C2 Double Nickase Plasmid (h) and SLC35C2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC35C2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLC35C2 Double Nickase Plasmid (h) | sc-409264-NIC | 20 µg | $410.00 |
SLC35C2 encodes a Golgi-resident nucleotide sugar transporter implicated in supplying activated monosaccharide donors required for protein and lipid glycosylation. By regulating nucleotide-sugar flux into the secretory pathway, SLC35C2 can influence glycan maturation, cell-surface glycoprotein composition, and trafficking-dependent processes such as adhesion and receptor signaling. Perturbation of Golgi glycosylation networks is broadly linked to altered immune recognition, developmental phenotypes, and stress responses, making SLC35C2 a useful target for dissecting glycosylation-dependent cell biology. Expression and functional variation in SLC35 family transporters are frequently explored in the context of congenital disorders of glycosylation and cancer-associated glycome remodeling, supporting mechanistic studies of pathway sensitivity and redundancy.
SLC35C2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC35C2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC35C2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC35C2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC35C2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.