SLC35A4 Double Nickase Plasmid (h): sc-414030-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLC35A4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SLC35A4 Double Nickase Plasmid (h) and SLC35A4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC35A4. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLC35A4 Double Nickase Plasmid (h) | sc-414030-NIC | 20 µg | $410.00 | |||
SLC35A4 Double Nickase Plasmid (h2) | sc-414030-NIC-2 | 20 µg | $410.00 |
SLC35A4 encodes a member of the solute carrier family 35 of nucleotide-sugar transporters, a group that supports luminal supply of activated sugars to the ER and Golgi for glycosylation reactions. Although SLC35A4 remains less well characterized than other SLC35 paralogs, it is implicated in regulating nucleotide-sugar homeostasis that influences glycoprotein and glycolipid biosynthesis, protein folding, and intracellular trafficking. Perturbation of nucleotide-sugar transport and downstream glycosylation networks can remodel cell-surface composition and signaling, affecting processes such as adhesion, receptor maturation, and immune recognition. Accordingly, SLC35A4 is of interest for mechanistic studies in glycomics and secretory pathway biology, and for investigating how altered glycosylation contributes to cellular stress responses and disease-associated phenotypes.
SLC35A4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC35A4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC35A4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC35A4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC35A4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.