SLC25A48 Double Nickase Plasmid (h): sc-414730-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLC25A48 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SLC25A48 Double Nickase Plasmid (h) and SLC25A48 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC25A48. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLC25A48 Double Nickase Plasmid (h) | sc-414730-NIC | 20 µg | $410.00 | |||
SLC25A48 Double Nickase Plasmid (h2) | sc-414730-NIC-2 | 20 µg | $410.00 |
SLC25A48 encodes a member of the mitochondrial solute carrier family implicated in transport processes across the inner mitochondrial membrane, linking metabolite exchange to oxidative phosphorylation efficiency and cellular energy balance. By shaping mitochondrial substrate availability and redox homeostasis, SLC25A48 is positioned to influence stress responses, metabolic remodeling, and cell-state transitions that depend on mitochondrial function. Altered regulation of mitochondrial carriers is frequently studied in contexts of metabolic disease, neurodegeneration, and cancer-associated bioenergetic rewiring, making SLC25A48 a useful node for mechanistic investigation. Functional interrogation of SLC25A48 can help clarify how mitochondrial transport interfaces with pathways controlling ATP production, reactive oxygen species management, and apoptotic sensitivity.
SLC25A48 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC25A48 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC25A48. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC25A48 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC25A48-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.