SLC25A36 Double Nickase Plasmid (m): sc-431417-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SLC25A36 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SLC25A36 Double Nickase Plasmid (m) and SLC25A36 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Slc25a36. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SLC25A36 Double Nickase Plasmid (m) | sc-431417-NIC | 20 µg | $410.00 | |||
SLC25A36 Double Nickase Plasmid (m2) | sc-431417-NIC-2 | 20 µg | $410.00 |
Slc25a36 encodes SLC25A36, a mitochondrial inner membrane solute carrier implicated in maintaining nucleotide homeostasis by mediating transport of pyrimidine nucleotides and related metabolites across the mitochondrial membrane. By regulating intramitochondrial nucleotide pools, SLC25A36 supports mitochondrial DNA replication and repair, oxidative phosphorylation capacity, and broader mitochondrial metabolic flux. Perturbation of mitochondrial nucleotide transport can disrupt bioenergetics and stress responses, processes linked to neurodevelopmental and neuromuscular phenotypes and other disorders associated with mitochondrial dysfunction. In mouse systems, Slc25a36 provides a tractable target for studying how nucleotide transport intersects with mitochondrial genome maintenance, redox balance, and cell-state transitions.
SLC25A36 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Slc25a36 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Slc25a36. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Slc25a36 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Slc25a36-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.