Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Ska2 CRISPR/Cas9 KO Plasmid (h): sc-405933
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ska2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ska2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Ska2 Antibody (H-2): sc-514495
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ska2 CRISPR/Cas9 KO Plasmid (h) | sc-405933 | 20 µg | $397.00 |
Overview
SKA2 encodes spindle and kinetochore associated complex subunit 2 (Ska2), a core component of the SKA complex that cooperates with the NDC80 complex to stabilize kinetochore–microtubule attachments during mitosis. Ska2 supports chromosome congression and anaphase onset by promoting persistent end-on microtubule binding and contributing to spindle checkpoint silencing. Through its role in mitotic progression, SKA2 influences cell-cycle dynamics, chromosome segregation fidelity, and genomic stability. Dysregulated SKA2 expression or function has been associated with proliferative phenotypes and chromosomal instability observed in multiple disease contexts, making it relevant for mechanistic studies of division-linked stress responses.
Ska2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SKA2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SKA2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SKA2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ska2 protein expression.
This CRISPR knockout system enables efficient generation of SKA2-deficient cell models for investigation of Ska2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SKA2 exon(s) critical for Ska2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SKA2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ska2 CRISPR/Cas9 KO Plasmid (h) and Ska2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SKA2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ska2 HDR Plasmid (h) and Ska2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SKA2 homology arms to support homology-directed repair at defined SKA2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.