SK-N-MC nuclear extract: sc-2154
- supplied in four vials, each containing 250 µg nuclear extract in 50 µl buffer
- provided in 20 mM HEPES (pH 7.9), 20% v/v glycerol, 0.1 M KCI, 0.2 mM EDTA, 0.5 mM PMSF and 0.5 mM DTT
- human nuclear extract; neuroepithelioma cells
- suitable for use in Gel Shift and Western Blotting assays
- Extracts should be stored at -70°C and repeated freezing and thawing should be avoided.
- prepared by the method of Dignam et al., (1983) Nucleic Acids Res. 11: 1475
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SK-N-MC nuclear extract, derived from the SK-N-MC cell line, which originates from human neuroepithelioma, is extensively utilized in neuroscience research. This extract contains a rich assortment of nuclear proteins, including transcription factors that are pivotal in studying neuronal differentiation, neurogenesis, and neuroplasticity. Researchers utilize SK-N-MC nuclear extract for a variety of assays such as electrophoretic mobility shift assays (EMSA) to analyze DNA-protein interactions, chromatin immunoprecipitation (ChIP) assays to investigate the binding of transcription factors to neuronal gene promoters, and co-immunoprecipitation experiments to study protein-protein interactions within the nucleus. These applications are crucial for understanding the regulatory mechanisms governing neuronal cell function and pathology in a purely scientific context. The use of this nuclear extract helps explain pathways involved in neural development and the molecular basis of neurobehavioral processes. Ensuring the origin and integrity of the SK-N-MC cell line is fundamental to maintaining the reliability and reproducibility of experimental results, thereby advancing our fundamental knowledge of neural cell biology.
SK-N-MC nuclear extract References:
- A novel glucocorticoid regulatory unit mediates the hormone responsiveness of the beta1-adrenergic receptor gene. | Tseng, YT., et al. 2001. Mol Cell Endocrinol. 181: 165-78. PMID: 11476950
- Neuronal specific regulatory elements in apolipoprotein E gene proximal promoter. | Ramos, MC., et al. 2005. Neuroreport. 16: 1027-30. PMID: 15931082
- CTIP2 associates with the NuRD complex on the promoter of p57KIP2, a newly identified CTIP2 target gene. | Topark-Ngarm, A., et al. 2006. J Biol Chem. 281: 32272-83. PMID: 16950772
- Differential IFN-gamma stimulation of HLA-A gene expression through CRM-1-dependent nuclear RNA export. | Browne, SK., et al. 2006. J Immunol. 177: 8612-9. PMID: 17142760
- Activator region analysis of the human D1A dopamine receptor gene. | Minowa, MT., et al. 1993. J Biol Chem. 268: 23544-51. PMID: 8226883
- Essential promoter elements are located within the 5′ untranslated region of human insulin-like growth factor-I exon I. | Mittanck, DW., et al. 1997. Mol Cell Endocrinol. 126: 153-63. PMID: 9089653
- An inverted cAMP response element mediates the cAMP induction of the ovine beta 1-adrenergic receptor gene. | Tseng, YT., et al. 1998. Biochem Mol Biol Int. 46: 1127-34. PMID: 9891845
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SK-N-MC nuclear extract | sc-2154 | 250 µg/0.05 ml | $160.00 |