SK-BR-3 + PMA nuclear extract: sc-2135

SK-BR-3+PMA nuclear extract combines the human breast cancer cell line SK-BR-3 with Phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C (PKC). This unique combination is used extensively in cancer biology research to study the effects of PKC activation on cancer cell signaling pathways. The extract is particularly valuable for investigating changes in transcription factor activity, gene expression, and nuclear protein interactions under conditions of induced PKC activation. Researchers employ SK-BR-3+PMA nuclear extract in various assays such as electrophoretic mobility shift assays (EMSA) to assess the binding of activated transcription factors to DNA, chromatin immunoprecipitation (ChIP) to explore changes in chromatin structure and gene expression, and co-immunoprecipitation to analyze protein-protein interactions that may influence cellular processes like proliferation and survival. These studies help in elucidating the molecular mechanisms by which PKC modulates signaling networks in cancer cells, contributing to a deeper understanding of cellular behavior. Verification of the SK-BR-3 cell line origin and the controlled use of PMA are critical to ensuring the accuracy and reproducibility of experimental results, focused entirely on advancing fundamental scientific inquiry.