SK-BR-3 nuclear extract: sc-2134

SK-BR-3 nuclear extract is derived from the SK-BR-3 cell line, which is a human breast cancer cell line extensively used in cancer biology research, particularly for studying the signaling pathways involved in the proliferation and survival of cancer cells. This nuclear extract is rich in transcription factors and other nuclear proteins that are key to understanding the regulation of gene expression related to cell growth and division. Researchers utilize SK-BR-3 nuclear extract in a variety of biochemical assays, such as electrophoretic mobility shift assays (EMSA) to identify DNA-protein interactions, chromatin immunoprecipitation (ChIP) assays to study the interaction of transcription factors with specific genomic regions, and co-immunoprecipitation experiments to explain complex protein-protein interactions within the nucleus. These studies contribute significantly to our understanding of the transcriptional mechanisms that may influence cell cycle dynamics and cellular responses to external stimuli in cancer cells. The extract enables detailed examination of cellular processes at the molecular level, facilitating advancements in basic science research. Verification of the origin and handling of the SK-BR-3 cell line is crucial for ensuring the authenticity and reliability of research outcomes, focusing purely on advancing scientific knowledge.

SK-BR-3 nuclear extract References:

1. Poly(ADP-ribose) polymerase and Ku autoantigen form a complex and synergistically bind to matrix attachment sequences.  |  Galande, S. and Kohwi-Shigematsu, T. 1999. J Biol Chem. 274: 20521-8. PMID: 10400681
2. Caught in the act: binding of Ku and PARP to MARs reveals novel aspects of their functional interaction.  |  Galande, S. and Kohwi-Shigematsu, T. 2000. Crit Rev Eukaryot Gene Expr. 10: 63-72. PMID: 10813395