SIV-1 Nef Antibody (04-001): sc-65911

SIV-1 Nef Antibody (04-001) is a mouse monoclonal IgG1 antibody that detects the SIV-1 Nef protein of SIV-1 origin by western blotting (WB), immunofluorescence (IF), and immunohistochemistry. Anti-SIV-1 Nef antibody (04-001) is available as the non-conjugated format. The simian immunodeficiency virus (SIV) is a retrovirus that affects primates and includes multiple strains, with HIV-1 and HIV-2 being the most relevant to humans. SIV-1 Nef protein, also known as the negative factor, plays a crucial role in the virus′s life cycle by optimizing the host cell environment for virion replication while simultaneously preventing apoptosis through the inhibition of Fas and TNFR-mediated death signals, as well as reducing the half-life of p53. Notably, the N-terminus of SIV-1 Nef contains myristoylation sites that anchor SIV-1 Nef to the cytoplasmic side of the plasma membrane upon activation, which is essential for function. SIV-1 Nef operates as a homodimer and interacts with various proteins, including PI 3-kinase, which activates PAK2 and enhances SIV production. Furthermore, SIV-1 Nef is instrumental in immune evasion, as SIV-1 Nef down-regulates MHC-I molecules and decreases CD4 antigens on the surface of infected cells, thereby allowing the virus to persist in the host.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SIV-1 Nef Antibody (04-001) References:

1. Two elements target SIV Nef to the AP-2 clathrin adaptor complex, but only one is required for the induction of CD4 endocytosis.  |  Lock, M., et al. 1999. EMBO J. 18: 2722-33. PMID: 10329619
2. Monoclonal antibodies recognize at least five epitopes on the SIV Nef protein and identify an in vitro-induced mutation.  |  Arnold, C., et al. 1999. AIDS Res Hum Retroviruses. 15: 1087-97. PMID: 10461829
3. Cutting edge: SIV Nef protein utilizes both leucine- and tyrosine-based protein sorting pathways for down-regulation of CD4.  |  Bresnahan, PA., et al. 1999. J Immunol. 163: 2977-81. PMID: 10477559
4. HIV-2 and SIV nef proteins target different Src family SH3 domains than does HIV-1 Nef because of a triple amino acid substitution.  |  Collette, Y., et al. 2000. J Biol Chem. 275: 4171-6. PMID: 10660579
5. Expression of simian immunodeficiency virus (SIV) nef in astrocytes during acute and terminal infection and requirement of nef for optimal replication of neurovirulent SIV in vitro.  |  Overholser, ED., et al. 2003. J Virol. 77: 6855-66. PMID: 12768005
6. Functional analysis of HIV1 and SIV Nef proteins.  |  Niederman, TM. and Ratner, L. 1992. Res Virol. 143: 43-6. PMID: 1565854
7. Conformational features of the full-length HIV and SIV Nef proteins determined by mass spectrometry.  |  Hochrein, JM., et al. 2006. Biochemistry. 45: 7733-9. PMID: 16784224
8. Activation of PAK by HIV and SIV Nef: importance for AIDS in rhesus macaques.  |  Sawai, ET., et al. 1996. Curr Biol. 6: 1519-27. PMID: 8939608
9. Structural and functional correlates between HIV-1 and SIV Nef isolates.  |  Garcia, JV. and Foster, JL. 1996. Virology. 226: 161-6. PMID: 8955034

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SIV-1 Nef Antibody (04-001) has 4 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SIV-1 Nef Antibody (04-001) Specifications

Alternate Product Name: SIV-1 Nef

Product Clone Name: 04-001

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: Nef

Epitope Species: SIV-1 (mac251strain)

Product Reactivity/Detection: amino acids 71-80 of Nef

Species Reactivity/Detection: SIV-1

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF, IHC(P)

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for SIV-1 Nef Antibody (04-001): N/A