SIP1/ZEB2 Double Nickase Plasmid (h): sc-400655-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SIP1/ZEB2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SIP1/ZEB2 Double Nickase Plasmid (h) and SIP1/ZEB2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZEB2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SIP1/ZEB2 Double Nickase Plasmid (h) | sc-400655-NIC | 20 µg | $410.00 | |||
SIP1/ZEB2 Double Nickase Plasmid (h2) | sc-400655-NIC-2 | 20 µg | $410.00 |
ZEB2 (also known as SIP1) encodes a zinc finger E-box–binding transcription factor that functions predominantly as a transcriptional repressor controlling epithelial–mesenchymal transition, lineage specification, and cell fate decisions. By binding E-box motifs and engaging CtBP/SMAD-associated corepressor complexes, SIP1/ZEB2 integrates TGF-β/BMP signaling with chromatin remodeling programs to modulate epithelial polarity genes, cell adhesion, and migratory phenotypes. ZEB2-dependent transcriptional networks contribute to neural crest development and immune cell differentiation, and dysregulation of ZEB2 expression or activity is linked to developmental syndromes and altered differentiation states in cancer-relevant contexts. These properties make ZEB2 a widely used node for mechanistic studies of transcriptional control, plasticity, and signaling-to-gene-expression coupling.
SIP1/ZEB2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZEB2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZEB2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZEB2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZEB2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.