Sigma 54 Antibody (33.7): sc-73492

Sigma 54 Antibody (33.7) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Sigma 54 protein of Aquifex aeolicus VF5 origin by western blotting (WB). Anti-Sigma 54 antibody (33.7) is available as a non-conjugated format, providing researchers with a reliable tool for studying this important protein. Sigma 54 is a crucial subunit of bacterial RNA polymerase (RNAP), playing a vital role in transcription activation when combined with other sigma factors. Sigma 54 binds to bacterial DNA at its C-terminus, functioning as a transcription regulator for sigma 54-dependent genes. The regulation of gene expression by Sigma 54 is particularly significant in environmental adaptability, as cellular levels can fluctuate throughout an organism′s lifecycle or in response to environmental stresses. For instance, in the plant pathogen Pseudomonas syringae, Sigma 54 is encoded by the rpoN gene, highlighting importance in pathogenicity and stress response. Additionally, in Pseudomonas alcaligenes, Sigma 54 is instrumental in regulating a wide array of genes, underscoring a role in bacterial adaptability and survival. Anti-Sigma 54 antibody (33.7) enables accurate detection and study of Sigma 54, essential for advancing understanding of bacterial transcription mechanisms and their implications in various biological processes.

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Sigma 54 Antibody (33.7) References:

1. Binding of transcriptional activators to sigma 54 in the presence of the transition state analog ADP-aluminum fluoride: insights into activator mechanochemical action.  |  Chaney, M., et al. 2001. Genes Dev. 15: 2282-94. PMID: 11544185
2. RpoN (sigma(54)) is required for plasmid-encoded coronatine biosynthesis in Pseudomonas syringae.  |  Alarcón-Chaidez, FJ., et al. 2003. Plasmid. 49: 106-17. PMID: 12726764
3. Mapping sigma 54-RNA polymerase interactions at the -24 consensus promoter element.  |  Burrows, PC., et al. 2003. J Biol Chem. 278: 29728-43. PMID: 12750380
4. Sigma 54 levels and physiological control of the Pseudomonas putida Pu promoter.  |  Jurado, P., et al. 2003. J Bacteriol. 185: 3379-83. PMID: 12754236
5. Enhancer-dependent transcription by bacterial RNA polymerase: the beta subunit downstream lobe is used by sigma 54 during open promoter complex formation.  |  Wigneshweraraj, SR., et al. 2003. Methods Enzymol. 370: 646-57. PMID: 14712681
6. Proteome investigation of the global regulatory role of sigma 54 in response to gentisate induction in Pseudomonas alcaligenes NCIMB 9867.  |  Zhao, B., et al. 2005. Proteomics. 5: 1868-76. PMID: 15815998
7. The sigma factor RpoN (sigma54) is involved in osmotolerance in Listeria monocytogenes.  |  Okada, Y., et al. 2006. FEMS Microbiol Lett. 263: 54-60. PMID: 16958851
8. Two chemosensory operons of Rhodobacter sphaeroides are regulated independently by sigma 28 and sigma 54.  |  Martin, AC., et al. 2006. J Bacteriol. 188: 7932-40. PMID: 16963577
9. Bacterial enhancer-binding proteins: unlocking sigma54-dependent gene transcription.  |  Rappas, M., et al. 2007. Curr Opin Struct Biol. 17: 110-6. PMID: 17157497

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Sigma 54 Antibody (33.7) has 0 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Sigma 54 Antibody (33.7) Specifications

Alternate Product Name: Sigma 54

Product Clone Name: 33.7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Sigma 54

Epitope Species: Aquifex aeolicus VF5

Product Reactivity/Detection: Sigma 54

Species Reactivity/Detection: Aquifex aeolicus VF5

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Sigma 54 Antibody (33.7): N/A