SIAH-1/2 Antibody (8G7H12): sc-81785

SIAH-1/2 Antibody (8G7H12) is a mouse monoclonal IgG1 antibody that detects SIAH-1/2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-SIAH-1/2 antibody (8G7H12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SIAH proteins, homologous to the Drosophila seven in absentia (sina) gene, function as E3 ubiquitin ligases and play a critical role in the ubiquitin-proteasome pathway, which regulates protein degradation and maintains cellular homeostasis. SIAH-1 interacts with several key proteins, including DCC (deleted in colorectal cancer) and Bag-1, influencing cell growth and apoptosis. SIAH-1′s ability to bind and regulate protein degradation underscores its importance as a tumor suppressor, helping control pathways that can lead to cancer progression when dysregulated. SIAH-1′s predominant nuclear localization suggests interaction with nuclear matrix proteins and DNA, further implicating a role in gene regulation and cellular stress responses. SIAH-1′s distinct structural features, including an N-terminal RING-finger domain necessary for ligase activity and a cysteine-rich C-terminal domain facilitating oligomerization, highlight the complexity of function and significance of post-translational modifications in activity modulation.

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SIAH-1/2 Antibody (8G7H12) References:

1. SIAH-1 promotes apoptosis and tumor suppression through a network involving the regulation of protein folding, unfolding, and trafficking: identification of common effectors with p53 and p21(Waf1).  |  Roperch, JP., et al. 1999. Proc Natl Acad Sci U S A. 96: 8070-3. PMID: 10393949
2. SIAH-1 inhibits cell growth by altering the mitotic process.  |  Bruzzoni-Giovanelli, H., et al. 1999. Oncogene. 18: 7101-9. PMID: 10597311
3. SIAH1 causes growth arrest and apoptosis in hepatoma cells through beta-catenin degradation-dependent and -independent mechanisms.  |  Yoshibayashi, H., et al. 2007. Oncol Rep. 17: 549-56. PMID: 17273732
4. Isolation of Xenopus HIF-prolyl 4-hydroxylase and rescue of a small-eye phenotype caused by Siah2 over-expression.  |  Imaoka, S., et al. 2007. Biochem Biophys Res Commun. 355: 419-25. PMID: 17303083
5. S100A6 binding protein and Siah-1 interacting protein (CacyBP/SIP): spotlight on properties and cellular function.  |  Schneider, G. and Filipek, A. 2011. Amino Acids. 41: 773-80. PMID: 20182755
6. The PINK1, synphilin-1 and SIAH-1 complex constitutes a novel mitophagy pathway.  |  Szargel, R., et al. 2016. Hum Mol Genet. 25: 3476-3490. PMID: 27334109
7. Interaction between the cellular E3 ubiquitin ligase SIAH-1 and the viral immediate-early protein ICP0 enables efficient replication of Herpes Simplex Virus type 2 in vivo.  |  Czechowicz, JS., et al. 2018. PLoS One. 13: e0201880. PMID: 30080903
8. Activation of the human homologue of the Drosophila sina gene in apoptosis and tumor suppression.  |  Nemani, M., et al. 1996. Proc Natl Acad Sci U S A. 93: 9039-42. PMID: 8799150
9. Mammalian homologs of seven in absentia regulate DCC via the ubiquitin-proteasome pathway.  |  Hu, G., et al. 1997. Genes Dev. 11: 2701-14. PMID: 9334332
10. Characterization of human homologs of the Drosophila seven in absentia (sina) gene.  |  Hu, G., et al. 1997. Genomics. 46: 103-11. PMID: 9403064
11. p53-inducible human homologue of Drosophila seven in absentia (Siah) inhibits cell growth: suppression by BAG-1.  |  Matsuzawa, S., et al. 1998. EMBO J. 17: 2736-47. PMID: 9582267
12. Siah-1 N-terminal RING domain is required for proteolysis function, and C-terminal sequences regulate oligomerization and binding to target proteins.  |  Hu, G. and Fearon, ER. 1999. Mol Cell Biol. 19: 724-32. PMID: 9858595

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SIAH-1/2 Antibody (8G7H12) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-SIAH-1/2 Antibody (8G7H12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SIAH-1/2 Antibody (8G7H12) Specifications

Alternate Product Name: E3 ligase SIAH (Seven In Absentia Homolog)

Product Clone Name: 8G7H12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: SIAH

Epitope Species: Drosophila melanogaster

Product Reactivity/Detection: SIAH-1 and SIAH-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine

Additional Non-Mammalian Reactivity: Drosophila

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for SIAH-1/2 Antibody (8G7H12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred SIAH-2 antibody is SIAH-2 Antibody (24E6H3): sc-81787.