SIAH-1/2 Antibody (4B4B6): sc-81786
- SIAH-1/2 Antibody (4B4B6) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against a synthetic peptide corresponding to a region near the C-terminus of SIAH of Drosophila melanogaster origin
- recommended for detection of SIAH-1 and SIAH-2 of human and Drosophila origin by WB, IF and IHC(P); also reactive with additional species, including porcine
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for SIAH-1/2 Antibody (4B4B6). This work is in progress.
SIAH-1/2 Antibody (4B4B6) is a mouse monoclonal IgG1 antibody that detects SIAH in human samples through applications such as western blotting (WB), immunofluorescence (IF), and immunohistochemistry. SIAH, the human homolog of the Drosophila seven in absentia (sina) gene, functions as a crucial tumor suppressor protein, primarily expressed in the intestinal epithelium and activated during apoptosis. The SIAH family consists of two distinct gene products, SIAH-1 and SIAH-2, which share a highly conserved C-terminal sequence but differ in their N-terminal regions. Notably, SIAH-1 features an N-terminal RING-finger domain essential for proteolytic activity, along with a cysteine-rich C-terminal domain that regulates oligomerization and binding to target proteins. As a tumor suppressor, SIAH-1 plays a pivotal role in cellular regulation by binding to the DCC (deleted in colorectal cancer) protein, facilitating degradation via the ubiquitin-proteasome pathway, and interacting with the Bcl-2 related protein Bag-1 to inhibit cell growth. SIAH-1 is primarily localized in the nucleus, with a smaller fraction found in the cytoplasm, indicating potential interactions with nuclear matrix proteins and DNA, which are critical for maintaining genomic stability and regulating apoptotic pathways. anti-SIAH-1/2 antibody (4B4B6) is an invaluable tool for researchers studying the mechanisms of tumor suppression and apoptosis in human tissues.
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SIAH-1/2 Antibody (4B4B6) References:
- The characterization of the human Siah-1 promoter(1). | Maeda, A., et al. 2002. FEBS Lett. 512: 223-6. PMID: 11852084
- Siah-1 facilitates ubiquitination and degradation of synphilin-1. | Nagano, Y., et al. 2003. J Biol Chem. 278: 51504-14. PMID: 14506261
- SIAH-1 interacts with CtIP and promotes its degradation by the proteasome pathway. | Germani, A., et al. 2003. Oncogene. 22: 8845-51. PMID: 14654780
- Siah-1 interacts with the intracellular region of polycystin-1 and affects its stability via the ubiquitin-proteasome pathway. | Kim, H., et al. 2004. J Am Soc Nephrol. 15: 2042-9. PMID: 15284290
- Inactivating mutations of the Siah-1 gene in gastric cancer. | Kim, CJ., et al. 2004. Oncogene. 23: 8591-6. PMID: 15467739
- Eukaryotic translation elongation factor 1 delta inhibits the ubiquitin ligase activity of SIAH-1. | Wu, H., et al. 2011. Mol Cell Biochem. 357: 209-15. PMID: 21633900
- E3 ubiquitin ligase Siah-1 downregulates synaptophysin expression under high glucose and hypoxia. | Zhao, Y., et al. 2015. Am J Transl Res. 7: 15-27. PMID: 25755825
- Siah-1 is associated with expression of hypoxia-inducible factor-1α in oral squamous cell carcinoma. | Aga, M., et al. 2017. Auris Nasus Larynx. 44: 213-219. PMID: 27616748
- The E3 Ubiquitin Ligase Siah-1 Suppresses Avian Reovirus Infection by Targeting p10 for Degradation. | Chen, X., et al. 2018. J Virol. 92: PMID: 29321312
- E3 ubiquitin ligase siah‑1 nuclear accumulation is critical for homocysteine‑induced impairment of C6 astroglioma cells. | Tian, X., et al. 2019. Mol Med Rep. 20: 2227-2235. PMID: 31322210
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SIAH-1/2 Antibody (4B4B6) | sc-81786 | 200 µg/ml | $322.00 |