SIAE CRISPR/Cas9 KO Plasmid (m): sc-423745
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SIAE CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SIAE genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SIAE CRISPR/Cas9 KO Plasmid (m) | sc-423745 | 20 µg | $397.00 |
Overview
Mouse SIAE (sialic acid acetylesterase) encodes a lysosomal/secreted esterase that removes O-acetyl groups from sialic acids, remodeling glycan structures on glycoproteins and glycolipids. By regulating the acetylation state of sialylated ligands, SIAE influences lectin-mediated recognition events and downstream signaling at the cell surface, with strong relevance to immune cell homeostasis. Altered sialic acid modifications are linked to changes in B-cell receptor signaling thresholds, tolerance checkpoints, and inflammatory responses, making Siae a useful target in studies of autoimmunity and immune dysregulation. SIAE-dependent glycan editing also intersects with pathways governing endosomal/lysosomal processing and receptor trafficking, supporting investigations in glycobiology and immunometabolism.
SIAE CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Siae gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Siae together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Siae open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SIAE protein expression.
This CRISPR knockout system enables efficient generation of Siae-deficient cell models for investigation of SIAE signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Siae exon(s) critical for SIAE function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Siae genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SIAE CRISPR/Cas9 KO Plasmid (m) and SIAE CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Siae locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SIAE HDR Plasmid (m) and SIAE HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Siae homology arms to support homology-directed repair at defined Siae target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.