SHIP-2 Antibody (E-2): sc-166641

SHIP-2 Antibody (E-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects SHIP-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SHIP-2 (E-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SHIP-2 protein plays a crucial role in cellular signaling pathways, particularly in the regulation of immune responses and cell growth. SHIP-2 hydrolyzes phosphatidylinositol 3,4,5-trisphosphate (PtdIns P3) to phosphatidylinositol 3,4-bisphosphate (PtdIns P2), which is essential for inhibiting cell proliferation and promoting cell cycle arrest, particularly in glioblastoma cells. This function helps maintain cellular homeostasis and prevents uncontrolled cell growth, which can lead to tumorigenesis. SHIP-2 is involved in the signaling pathways of various immune cells, including T and B lymphocytes, and SHIP-2 expression is modulated by factors such as macrophage colony-stimulating factor (M-CSF) and epidermal growth factor (EGF). SHIP-2 interaction with other proteins, such as Shc, further underscores SHIP-2′s importance in mediating cellular responses to external stimuli. With diverse roles in both hematopoietic and non-hematopoietic cells, SHIP-2 is a significant player in the regulation of immune functions and cellular signaling, making anti-SHIP-2 antibody (E-2) an invaluable tool for researchers studying these critical biological processes.

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SHIP-2 Antibody (E-2) References:

1. Distribution of the src-homology-2-domain-containing inositol 5-phosphatase SHIP-2 in both non-haemopoietic and haemopoietic cells and possible involvement of SHIP-2 in negative signalling of B-cells.  |  Muraille, E., et al. 1999. Biochem J. 342 Pt 3: 697-705. PMID: 10477282
2. CD28 stimulates tyrosine phosphorylation, cellular redistribution and catalytic activity of the inositol lipid 5-phosphatase SHIP.  |  Edmunds, C., et al. 1999. Eur J Immunol. 29: 3507-15. PMID: 10556805
3. 5' phospholipid phosphatase SHIP-2 causes protein kinase B inactivation and cell cycle arrest in glioblastoma cells.  |  Taylor, V., et al. 2000. Mol Cell Biol. 20: 6860-71. PMID: 10958682
4. Cloning of the genomic locus of mouse SH2 containing inositol 5-phosphatase (SHIP) and a novel 110-kDa splice isoform, SHIPdelta.  |  Wolf, I., et al. 2000. Genomics. 69: 104-12. PMID: 11013080
5. Partially distinct molecular mechanisms mediate inhibitory FcgammaRIIB signaling in resting and activated B cells.  |  Brauweiler, A., et al. 2001. J Immunol. 167: 204-11. PMID: 11418650
6. Descending-SHIP2-mediated radiosensitivity enhancement through PI3K/Akt signaling pathway in laryngeal squamous cell carcinoma.  |  Tang, T., et al. 2019. Biomed Pharmacother. 118: 109392. PMID: 31545285
7. Upregulation of SHIP2 participates in the development of breast cancer via promoting Wnt/β-catenin signaling.  |  Zhou, J., et al. 2019. Onco Targets Ther. 12: 7067-7077. PMID: 31564892
8. Scaffold dependent role of the inositol 5'-phosphatase SHIP2, in regulation of oxidative stress induced apoptosis.  |  Azzi, A. 2021. Arch Biochem Biophys. 697: 108667. PMID: 33181128
9. Metformin Effects on SHIP2, AMPKs and Gut Microbiota: Recent Updates on Pharmacology.  |  Shivaprakash, P., et al. 2024. Curr Med Chem.. PMID: 38409699
10. p150Ship, a signal transduction molecule with inositol polyphosphate-5-phosphatase activity.  |  Lioubin, MN., et al. 1996. Genes Dev. 10: 1084-95. PMID: 8654924
11. The Src homology 2 (SH2) domain of SH2-containing inositol phosphatase (SHIP) is essential for tyrosine phosphorylation of SHIP, its association with Shc, and its induction of apoptosis.  |  Liu, L., et al. 1997. J Biol Chem. 272: 8983-8. PMID: 9083021
12. Identification of a second SH2-domain-containing protein closely related to the phosphatidylinositol polyphosphate 5-phosphatase SHIP.  |  Pesesse, X., et al. 1997. Biochem Biophys Res Commun. 239: 697-700. PMID: 9367831

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SHIP-2 Antibody (E-2) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-SHIP-2 Antibody (E-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SHIP-2 Antibody (E-2) Specifications

Alternate Product Name: SHIP-2

Product Clone Name: E-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 959-1258

Epitope Target: SHIP-2

Epitope Species: human

Product Reactivity/Detection: SHIP-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for SHIP-2 Antibody (E-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin