Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Shc Double Nickase Plasmid (h): sc-400914-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Shc Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Shc Double Nickase Plasmid (h) and Shc Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SHC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Shc Antibody (PG-797): sc-967
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Shc Double Nickase Plasmid (h) | sc-400914-NIC | 20 µg | $410.00 | |||
Shc Double Nickase Plasmid (h2) | sc-400914-NIC-2 | 20 µg | $410.00 |
SHC1 encodes the adaptor protein Shc, a key signaling intermediate that couples activated receptor tyrosine kinases and cytokine receptors to downstream cascades controlling proliferation, survival, differentiation, and stress responses. Through its PTB and SH2 domains, Shc engages phosphorylated receptors and recruits GRB2/SOS to promote RAS–RAF–MEK–ERK signaling, and can also interface with PI3K/AKT and other MAPK pathways depending on stimulus and cell type. SHC1 participates in growth factor signaling dynamics, receptor trafficking, and mitogenic transcriptional programs, making it relevant to studies of oncogenic signaling rewiring and resistance mechanisms. Altered SHC1 expression or phosphorylation has been associated with aberrant pathway activation in cancer and with dysregulated cellular responses in metabolic and inflammatory contexts.
Shc Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SHC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SHC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SHC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SHC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.