SET7/9 Antibody (C-11): sc-390823

SET7/9 Antibody (C-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects SET7/9 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SET7/9 (C-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SET7/9, a histone methyltransferase, plays a crucial role in gene expression regulation through methylating lysine 4 on histone H3, a modification essential for transcription activation. This methylation event is vital for chromatin remodeling and influences transcriptional machinery recruitment, impacting cellular processes such as differentiation and development. SET7/9 operates through a complex mechanism involving S-adenosyl-L-methionine, recognizing specific lysine residues in histones to ensure precise gene expression regulation. Unique structural features of SET7/9, including binding pocket and substrate-specific channel, allow selective interaction with unmethylated lysine residues, underscoring SET7/9′s importance in epigenetic regulation and cellular function.

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SET7/9 Antibody (C-11) References:

1. Re-SET-ting heterochromatin by histone methyltransferases.  |  Jenuwein, T. 2001. Trends Cell Biol. 11: 266-73. PMID: 11356363
2. Purification and functional characterization of a histone H3-lysine 4-specific methyltransferase.  |  Wang, H., et al. 2001. Mol Cell. 8: 1207-17. PMID: 11779497
3. Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation.  |  Nishioka, K., et al. 2002. Genes Dev. 16: 479-89. PMID: 11850410
4. Crystal structure and functional analysis of the histone methyltransferase SET7/9.  |  Wilson, JR., et al. 2002. Cell. 111: 105-15. PMID: 12372304
5. Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet.  |  Kwon, T., et al. 2003. EMBO J. 22: 292-303. PMID: 12514135
6. Structure and catalytic mechanism of the human histone methyltransferase SET7/9.  |  Xiao, B., et al. 2003. Nature. 421: 652-6. PMID: 12540855
7. Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1.  |  Wysocka, J., et al. 2003. Genes Dev. 17: 896-911. PMID: 12670868
8. Gene-specific modulation of TAF10 function by SET9-mediated methylation.  |  Kouskouti, A., et al. 2004. Mol Cell. 14: 175-82. PMID: 15099517
9. SET7/9 promotes hepatocellular carcinoma progression through regulation of E2F1.  |  Gu, Y., et al. 2018. Oncol Rep. 40: 1863-1874. PMID: 30106440
10. SET7/9 promotes H3K4me3 at lncRNA DRAIC promoter to modulate growth and metastasis of glioma.  |  Li, C., et al. 2020. Eur Rev Med Pharmacol Sci. 24: 12241-12250. PMID: 33336743
11. Oncogene or Tumor Suppressor: The Coordinative Role of Lysine Methyltransferase SET7/9 in Cancer Development and the Related Mechanisms.  |  Gu, Y., et al. 2022. J Cancer. 13: 623-640. PMID: 35069908
12. Combined SET7/9 and CDK4 inhibition act synergistically against osteosarcoma.  |  Shi, Y., et al. 2024. Biochem Biophys Res Commun. 708: 149808. PMID: 38520914

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SET7/9 Antibody (C-11) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-SET7/9 Antibody (C-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SET7/9 Antibody (C-11) Specifications

Alternate Product Name: SET7/9

Product Clone Name: C-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: SET7/9

Epitope Species: human

Product Reactivity/Detection: SET7/9

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for SET7/9 Antibody (C-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin