SET7/9 Antibody (C-11): sc-390823
- SET7/9 Antibody (C-11) is a mouse monoclonal IgG1 κ SET7/9 antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 1-300 mapping at the N-terminus of SET7/9 of human origin
- SET7/9 Antibody (C-11) is recommended for detection of SET7/9 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-SET7/9 Antibody (C-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for SET7/9 Antibody (C-11) for WB applications. This reagent is now offered in a bundle with SET7/9 Antibody (C-11) (see ordering information below).
SET7/9 Antibody (C-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects SET7/9 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SET7/9 (C-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SET7/9, a histone methyltransferase, plays a crucial role in gene expression regulation through methylating lysine 4 on histone H3, a modification essential for transcription activation. This methylation event is vital for chromatin remodeling and influences transcriptional machinery recruitment, impacting cellular processes such as differentiation and development. SET7/9 operates through a complex mechanism involving S-adenosyl-L-methionine, recognizing specific lysine residues in histones to ensure precise gene expression regulation. Unique structural features of SET7/9, including binding pocket and substrate-specific channel, allow selective interaction with unmethylated lysine residues, underscoring SET7/9′s importance in epigenetic regulation and cellular function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SET7/9 Antibody (C-11) References:
- Re-SET-ting heterochromatin by histone methyltransferases. | Jenuwein, T. 2001. Trends Cell Biol. 11: 266-73. PMID: 11356363
- Purification and functional characterization of a histone H3-lysine 4-specific methyltransferase. | Wang, H., et al. 2001. Mol Cell. 8: 1207-17. PMID: 11779497
- Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation. | Nishioka, K., et al. 2002. Genes Dev. 16: 479-89. PMID: 11850410
- Crystal structure and functional analysis of the histone methyltransferase SET7/9. | Wilson, JR., et al. 2002. Cell. 111: 105-15. PMID: 12372304
- Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet. | Kwon, T., et al. 2003. EMBO J. 22: 292-303. PMID: 12514135
- Structure and catalytic mechanism of the human histone methyltransferase SET7/9. | Xiao, B., et al. 2003. Nature. 421: 652-6. PMID: 12540855
- Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1. | Wysocka, J., et al. 2003. Genes Dev. 17: 896-911. PMID: 12670868
- Gene-specific modulation of TAF10 function by SET9-mediated methylation. | Kouskouti, A., et al. 2004. Mol Cell. 14: 175-82. PMID: 15099517
- SET7/9 promotes hepatocellular carcinoma progression through regulation of E2F1. | Gu, Y., et al. 2018. Oncol Rep. 40: 1863-1874. PMID: 30106440
- SET7/9 promotes H3K4me3 at lncRNA DRAIC promoter to modulate growth and metastasis of glioma. | Li, C., et al. 2020. Eur Rev Med Pharmacol Sci. 24: 12241-12250. PMID: 33336743
- Oncogene or Tumor Suppressor: The Coordinative Role of Lysine Methyltransferase SET7/9 in Cancer Development and the Related Mechanisms. | Gu, Y., et al. 2022. J Cancer. 13: 623-640. PMID: 35069908
- Combined SET7/9 and CDK4 inhibition act synergistically against osteosarcoma. | Shi, Y., et al. 2024. Biochem Biophys Res Commun. 708: 149808. PMID: 38520914
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SET7/9 Antibody (C-11) | sc-390823 | 200 µg/ml | $322.00 | |||
SET7/9 Antibody (C-11): m-IgG1 BP-HRP Bundle | sc-531743 | 200 µg Ab; 20 µg BP | $361.00 | |||
SET7/9 Antibody (C-11) AC | sc-390823 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SET7/9 Antibody (C-11) HRP | sc-390823 HRP | 200 µg/ml | $322.00 | |||
SET7/9 Antibody (C-11) FITC | sc-390823 FITC | 200 µg/ml | $336.00 | |||
SET7/9 Antibody (C-11) PE | sc-390823 PE | 200 µg/ml | $349.00 | |||
SET7/9 Antibody (C-11) Alexa Fluor® 488 | sc-390823 AF488 | 200 µg/ml | $364.00 | |||
SET7/9 Antibody (C-11) Alexa Fluor® 546 | sc-390823 AF546 | 200 µg/ml | $364.00 | |||
SET7/9 Antibody (C-11) Alexa Fluor® 594 | sc-390823 AF594 | 200 µg/ml | $364.00 | |||
SET7/9 Antibody (C-11) Alexa Fluor® 647 | sc-390823 AF647 | 200 µg/ml | $364.00 | |||
SET7/9 Antibody (C-11) Alexa Fluor® 680 | sc-390823 AF680 | 200 µg/ml | $364.00 | |||
SET7/9 Antibody (C-11) Alexa Fluor® 790 | sc-390823 AF790 | 200 µg/ml | $364.00 |