Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SESN2 Double Nickase Plasmid (h): sc-402442-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SESN2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SESN2 Double Nickase Plasmid (h) and SESN2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SESN2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SESN2 Antibody (D-4): sc-393195
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SESN2 Double Nickase Plasmid (h) | sc-402442-NIC | 20 µg | $410.00 | |||
SESN2 Double Nickase Plasmid (h2) | sc-402442-NIC-2 | 20 µg | $410.00 |
SESN2 (sestrin-2) is a stress-inducible cytoprotective protein that integrates oxidative stress signaling with metabolic homeostasis. It modulates AMPK–mTORC1 signaling, supports autophagy regulation, and contributes to redox control through effects on ROS handling and antioxidant responses. SESN2 is induced downstream of stress-responsive transcriptional programs and participates in feedback regulation of nutrient sensing and cellular adaptation to DNA damage, hypoxia, and inflammatory cues. Dysregulated SESN2 activity has been linked to altered proteostasis and metabolism in contexts relevant to cancer biology, neurodegeneration, and cardiometabolic disease research.
SESN2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SESN2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SESN2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SESN2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SESN2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.