SerpinA9 Double Nickase Plasmid (h): sc-415475-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SerpinA9 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SerpinA9 Double Nickase Plasmid (h) and SerpinA9 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SERPINA9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SerpinA9 Antibody (913G2V): sc-517654
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SerpinA9 Double Nickase Plasmid (h) | sc-415475-NIC | 20 µg | $410.00 |
SERPINA9 encodes SerpinA9, a member of the serine protease inhibitor (serpin) superfamily that helps shape extracellular proteolysis by modulating protease activity in tissue microenvironments. Serpin-mediated control of protease cascades contributes to processes such as extracellular matrix remodeling, cell migration, and immune cell dynamics, linking SERPINA9 to pathways that influence inflammatory signaling and protease-dependent regulation of cellular communication. Altered expression patterns of SERPINA9 have been reported in lymphoid contexts, supporting its relevance for studies of B-cell biology, tumor microenvironment interactions, and immune-associated dysregulation. As a human protein-coding gene with tissue- and lineage-associated expression, SERPINA9 is frequently investigated in transcriptomic and functional genomic analyses to clarify its role in protease balance and immune homeostasis.
SerpinA9 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SERPINA9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SERPINA9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SERPINA9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SERPINA9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.