Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SERCA3 Double Nickase Plasmid (h): sc-402840-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SERCA3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SERCA3 Double Nickase Plasmid (h) and SERCA3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ATP2A3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SERCA3 Antibody (PL/IM430): sc-81759
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SERCA3 Double Nickase Plasmid (h) | sc-402840-NIC | 20 µg | $410.00 | |||
SERCA3 Double Nickase Plasmid (h2) | sc-402840-NIC-2 | 20 µg | $410.00 |
ATP2A3 encodes the human sarco/endoplasmic reticulum Ca2+-ATPase SERCA3, a P-type ATPase that pumps cytosolic Ca2+ into the ER lumen to restore basal calcium levels after signaling events. By controlling ER Ca2+ stores and cytosolic Ca2+ transients, SERCA3 helps shape calcium-dependent pathways including store-operated calcium entry, protein folding and quality control within the ER, and stimulus-secretion coupling in specialized cell types. Altered ATP2A3/SERCA3 expression or activity has been linked to dysregulated calcium homeostasis, ER stress responses, and changes in differentiation programs, with relevance to studies of immune cell signaling and epithelial biology. These functions make ATP2A3 a useful target for dissecting calcium handling mechanisms that influence proliferation, apoptosis, and inflammatory signaling without implying clinical outcomes.
SERCA3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ATP2A3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ATP2A3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ATP2A3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ATP2A3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.