Western blot analysis of c-Src phosphorylation in untreated (A,D), Ser/Thr induction cocktail (sc-362324) treated (B,E) and Ser/Thr induction cocktail (sc-362324) and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-c-Src (Thr 420)-R: sc-16845-R (A,B,C) and c-Src (B-12): sc-8056 (D,E,F).
Ser/Thr Phosphorylation Induction Cocktail
Application:
Ser/Thr Phosphorylation Induction Cocktail is a combination of chemicals that promotes and maintains the Ser/Thr phosphorylation state of a variety of proteins
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
Ser/Thr Phosphorylation Induction Cocktail is a synergistic combination of chemicals designed to promote and maintain the phosphorylation state of a wide variety of phosphorylated proteins. Ser/Thr Phosphorylation Induction Cocktail is a tested and verified proprietary formula containing PMA, forskolin, calyculin A, and okadaic acid specifically designed for broad-spectrum protein phosphorylation research. Ser/Thr Phosphorylation Induction Cocktail has been shown to activate PKC, PKA, and inhibit Ser/Thr phosphatases 1 and 2A. Suitable for use in Western Blotting, Flow Cytometry, and ImmunoFluorescence applications.
Ser/Thr Phosphorylation Induction Cocktail References
- Phorbol ester stimulates a protein kinase C-mediated agatoxin-TK-sensitive calcium permeability pathway in human red blood cells. | Andrews, DA., et al. 2002. Blood. 100: 3392-9. PMID: 12384442
- Regulation of choline transporter surface expression and phosphorylation by protein kinase C and protein phosphatase 1/2A. | Gates, J., et al. 2004. J Pharmacol Exp Ther. 310: 536-45. PMID: 15064333
- Protein kinase C-mediated phosphorylation of the calcium-sensing receptor is stimulated by receptor activation and attenuated by calyculin-sensitive phosphatase activity. | Davies, SL., et al. 2007. J Biol Chem. 282: 15048-56. PMID: 17376781
- Functional study of the effect of phosphatase inhibitors on KCNQ4 channels expressed in Xenopus oocytes. | Su, TR., et al. 2009. Acta Pharmacol Sin. 30: 1220-6. PMID: 19701239
- Inhibition of serine/threonine protein phosphatases enhances agonist-stimulated cAMP accumulation in UMR 106 osteoblast-like cells. | Kovacs, CS., et al. 1995. Mol Cell Endocrinol. 110: 9-16. PMID: 7545625
- Spontaneous Mn2+ entry is specifically inhibited by calyculin A in human leukemic HL-60 cells. | Leung, YM., et al. 1997. Eur J Pharmacol. 331: 337-40. PMID: 9274998
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ser/Thr Phosphorylation Induction Cocktail, 2 ml | sc-362324 | 2 ml | $241.00 |