Septin 4 Double Nickase Plasmid (h): sc-402288-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Septin 4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Septin 4 Double Nickase Plasmid (h) and Septin 4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SEPT4. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Septin 4 Double Nickase Plasmid (h) | sc-402288-NIC | 20 µg | $410.00 | |||
Septin 4 Double Nickase Plasmid (h2) | sc-402288-NIC-2 | 20 µg | $410.00 |
SEPT4 encodes septin 4, a GTP-binding cytoskeletal protein that assembles into hetero-oligomeric filaments and acts as a diffusion barrier and scaffold at membranes. Septin 4 contributes to cytokinesis, cell polarity, vesicle trafficking, and organization of the actin and microtubule cytoskeleton, supporting processes such as mitotic progression and compartmentalization of signaling complexes. In human cells, altered septin organization and SEPT4 expression have been linked to defects in cell division and cytoskeletal dynamics that are relevant to cancer biology and neurodegeneration research. SEPT4 has also been studied in apoptosis-associated signaling and in the context of protein aggregation and cellular stress responses.
Septin 4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SEPT4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SEPT4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SEPT4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SEPT4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.