Septin 11 Double Nickase Plasmid (h): sc-407969-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Septin 11 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Septin 11 Double Nickase Plasmid (h) and Septin 11 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SEPT11. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Septin 11 Double Nickase Plasmid (h) | sc-407969-NIC | 20 µg | $410.00 | |||
Septin 11 Double Nickase Plasmid (h2) | sc-407969-NIC-2 | 20 µg | $410.00 |
SEPT11 encodes Septin 11, a GTP-binding cytoskeletal protein that assembles into hetero-oligomeric septin filaments and ring-like structures that act as diffusion barriers and scaffolds at membranes. Septin 11 contributes to actin–microtubule coordination during cytokinesis, cell polarization, vesicle trafficking, and neurite morphology, integrating with pathways that govern cortical organization and cell division fidelity. Altered septin network dynamics and SEPT11 dysregulation have been investigated in contexts of aberrant proliferation, migration, and neurobiology, where cytoskeletal remodeling and membrane compartmentalization are central. These features make SEPT11 a useful target for probing cytoskeletal pathway crosstalk, membrane-associated signaling, and cell-cycle–linked structural checkpoints in human cell models.
Septin 11 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SEPT11 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SEPT11. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SEPT11 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SEPT11-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.