Sephadex® G-50 Fine is a suitable medium for batch treatments such as desalting and buffer exchange of biomolecules >30 000 molecular weight. The Fine small bead size gives Sephadex® G-50 Fine higher efficiency. Sephadex® G-50 Fine is suitable for purification of DNA from small molecules by gel filtration (exclusion limit: 20 bp dsDNA (rA)20). Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. Sephadex® G-50 has been used to purify PCR products prior to DNA sequencing to improve the sequencing quality.
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