Sephadex® G-50 Fine CAS: 9048-71-9

Sephadex® G-50 Fine (CAS 9048-71-9)

Sephadex G-50 Fine | CAS 9048-71-9 is rated 5.0 out of 5 by 1.
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Alternate Names: Sephadex® G-50 Fine is known as a gel filtration medium with Fine sized beading.
Application: Sephadex® G-50 Fine is an established gel filtration medium used for desalting and biomolecule buffer exchange.
CAS Number: 9048-71-9
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Sephadex® G-50 Fine is a suitable medium for batch treatments such as desalting and buffer exchange of biomolecules >30 000 molecular weight. The Fine small bead size gives Sephadex® G-50 Fine higher efficiency. Sephadex® G-50 Fine is suitable for purification of DNA from small molecules by gel filtration (exclusion limit: 20 bp dsDNA (rA)20). Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. Sephadex® G-50 has been used to purify PCR products prior to DNA sequencing to improve the sequencing quality.

Sephadex® is a registered trademark of GE Healthcare


References

1. Wilson, M. Barbara, and Paul K. Nakane. "The covalent coupling of proteins to periodate-oxidized sephadex: a new approach to immunoadsorbent preparation." Journal of immunological methods 12.1-2 (1976): 171-181.
2. Leonowicz, Andrzej, Grazyna Szklarz, and Maria Wojtaś-Wasilewska. "The effect of fungal laccase on fractionated lignosulphonates (Peritan Na)." Phytochemistry 24.3 (1985): 393-396.
3. Meezan, Elias, et al. "Comparative studies on the carbohydrate-containing membrane components of normal and virus-transformed mouse fibroblasts. II. Separation of glycoproteins and glycopeptides by sephadex chromatography." Biochemistry 8.6 (1969): 2518-2524.

Usage :
1 g of Sephadex® G-50 swells to 9-11 mL.

Matrix:Cross-linked dextran

Particle Size Wet:34 µm-208 µm

Partical Size Dry:20 µm-80 µm

pH Stability Working Range:2-10

pH stability, CIP:2–13

pH Stability Cleaning-in-Place (CIP):2-13

Flow Rate:< Darcy′s Law

Pressure/Flow Specification:min 150 cm/h, bed height 10 cm, column 5 cm i.d.

Fractionation [Mr] Globular Proteins:1 x 103 – 3 x 104

Fractionation [Mr] Dextrans:5 x 102-1 x 104

Exclusion Limit:> 3 x 104

Storage Conditions:4 to 30°C

Autoclavable:In wet form (pH 7.0) at 120°C for 30 min
Formulation :
1 g swells to 9-11 mL
Appearance :
Powder
Physical State :
Solid
Solubility :
Insoluble in water.
Storage :
Desiccate at room temperature
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
MDL Number :
MFCD00132771

Download SDS (MSDS)

Certificate of Analysis

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Dear Whom it may concern, Good Day! I would like to ask if you do deliver products to Malaysia? If I am interested with Sephadex&reg; G-50 Fine, 100g package size what is the price you can provide for 200 bottles?

Asked by: Tan Ting Ting
Thank you for your question. We can only provide this product, sc-255033: Sephadex&reg; G-50 Fine (CAS 9048-71-9), in the 25 gram size for use in research applications. We do deliver products to Malaysia. You can order directly, or you can contact one of our authorized distributors. A link to search for our distributors on our website is below. https://www.scbt.com/scbt/resources/distributors
Answered by: Technical Support
Date published: 2018-03-13
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Rated 5 out of 5 by from Various publications cite the use of Sephadex G Various publications cite the use of Sephadex G-50 Fine for batch treatments such as desalting. -SCBT Publication Review
Date published: 2015-03-15
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