Sephadex^® G-25 Fine (CAS 9041-35-4)

Sephadex® G-25 Fine, a cross-linked dextran gel, is predominantly utilized in biochemical research for size-exclusion chromatography, offering a method to purify and fractionate biomolecules based on their size and molecular weight. This chemical′s structure forms a matrix of controlled pore sizes, making it ideal for desalting and buffer exchange in protein and nucleic acid research, where separation of small molecules from larger biomolecules is required without affecting the biological activity of the latter. Researchers employ Sephadex® G-25 Fine extensively to study protein-protein interactions, enzyme kinetics, and other biochemical pathways by allowing the rapid removal of low molecular weight compounds from reaction mixtures. Additionally, it is used in the preparation of samples for further analysis by more sensitive techniques such as mass spectrometry and nuclear magnetic resonance (NMR) spectroscopy. This specificity and efficiency make Sephadex® G-25 Fine an indispensable tool in the lab, facilitating the exploration of complex biological systems and the purification of biochemical compounds essential for detailed scientific studies. Through these applications, it contributes fundamentally to the advancement of biochemical research, focusing purely on scientific inquiries.