Sephadex^® G-100, medium (CAS 9050-94-6)

Sephadex® G-100, medium, is a well-known size-exclusion chromatography medium frequently used in the purification and fractionation of biomolecules such as proteins, nucleic acids, and other macromolecules. This medium consists of cross-linked dextran beads, which serve as a molecular sieve, allowing for the separation of substances based on their size and shape. The mechanism of action of Sephadex® G-100 relies on its porous structure; molecules larger than the pores are excluded from entering and thus elute earlier from the column, while smaller molecules penetrate into the pores and have a longer path and elute later. This property makes it particularly effective for desalting, buffer exchange, and the removal of small contaminants from larger valuable biomolecules. In research settings, Sephadex® G-100 has been extensively utilized for studying protein-protein interactions, enzyme purification, and the analysis of complex biological samples where selective size filtration can explain structural and functional characteristics of the biomolecules involved. Its use in biochemistry and molecular biology labs is critical for applications where high-resolution separation of components based on size is necessary to achieve precise analytical results and maintain the integrity of sensitive biological samples.