Separase Antibody (B-4): sc-390314
- Separase Antibody (B-4) is a mouse monoclonal IgG2a κ Separase antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1496-1795 mapping at the C-terminus of Separase of human origin
- Separase Antibody (B-4) is recommended for detection of Separase of human origin by WB, IP, IF and ELISA
- Anti-Separase Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP is the preferred secondary detection reagent for Separase Antibody (B-4) for WB applications. This reagent is now offered in a bundle with Separase Antibody (B-4) (see ordering information below).
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Separase Antibody (B-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Separase protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Separase antibody (B-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Separase is a crucial cysteine protease that plays a vital role in regulating cell cycle, specifically in triggering anaphase by facilitating sister chromatid separation during mitosis. Separase maintains genomic stability by ensuring each daughter cell receives an accurate complement of chromosomes. Separase achieves this by hydrolyzing the SCC1 subunit of cohesin, a protein complex that holds sister chromatids together. During non-division phases, Separase remains inhibited through association with securin. Upon anaphase onset, securin undergoes degradation by the anaphase-promoting complex/cyclosome, releasing active Separase. Separase also activates transiently during meiosis and may contribute to homologous chromosome separation, highlighting a significant role in both mitotic and meiotic cell divisions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Separase Antibody (B-4) References:
- Regulation of human separase by securin binding and autocleavage. | Waizenegger, I., et al. 2002. Curr Biol. 12: 1368-78. PMID: 12194817
- Anaphase specific auto-cleavage of separase. | Zou, H., et al. 2002. FEBS Lett. 528: 246-50. PMID: 12297314
- Mitotic regulation: the fine tuning of separase activity. | Agarwal, R. and Cohen-Fix, O. 2002. Cell Cycle. 1: 255-7. PMID: 12429942
- A non-proteolytic function of separase links the onset of anaphase to mitotic exit. | Sullivan, M. and Uhlmann, F. 2003. Nat Cell Biol. 5: 249-54. PMID: 12598903
- Western blot screening for monoclonal antibodies against human separase. | Chestukhin, A. and DeCaprio, JA. 2003. J Immunol Methods. 274: 105-13. PMID: 12609537
- Processing, localization, and requirement of human separase for normal anaphase progression. | Chestukhin, A., et al. 2003. Proc Natl Acad Sci U S A. 100: 4574-9. PMID: 12672959
- Separase is required at multiple pre-anaphase cell cycle stages in human cells. | Giménez-Abián, JF., et al. 2005. Cell Cycle. 4: 1576-84. PMID: 16177575
- Phosphorylation-dependent binding of cyclin B1 to a Cdc6-like domain of human separase. | Boos, D., et al. 2008. J Biol Chem. 283: 816-23. PMID: 17974570
- The complete removal of cohesin from chromosome arms depends on separase. | Nakajima, M., et al. 2007. J Cell Sci. 120: 4188-96. PMID: 18003702
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Separase Antibody (B-4) | sc-390314 | 200 µg/ml | $322.00 | |||
Separase Antibody (B-4): m-IgG2a BP-HRP Bundle | sc-545961 | 200 µg Ab; 10 µg BP | $361.00 | |||
Separase Antibody (B-4) AC | sc-390314 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Separase Antibody (B-4) HRP | sc-390314 HRP | 200 µg/ml | $322.00 | |||
Separase Antibody (B-4) FITC | sc-390314 FITC | 200 µg/ml | $336.00 | |||
Separase Antibody (B-4) PE | sc-390314 PE | 200 µg/ml | $349.00 | |||
Separase Antibody (B-4) Alexa Fluor® 488 | sc-390314 AF488 | 200 µg/ml | $364.00 | |||
Separase Antibody (B-4) Alexa Fluor® 546 | sc-390314 AF546 | 200 µg/ml | $364.00 | |||
Separase Antibody (B-4) Alexa Fluor® 594 | sc-390314 AF594 | 200 µg/ml | $364.00 | |||
Separase Antibody (B-4) Alexa Fluor® 647 | sc-390314 AF647 | 200 µg/ml | $364.00 | |||
Separase Antibody (B-4) Alexa Fluor® 680 | sc-390314 AF680 | 200 µg/ml | $364.00 | |||
Separase Antibody (B-4) Alexa Fluor® 790 | sc-390314 AF790 | 200 µg/ml | $364.00 |