Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SENP7 CRISPR/Cas9 KO Plasmid (h): sc-405886
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SENP7 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SENP7 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SENP7 Antibody (E-8): sc-373821
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SENP7 CRISPR/Cas9 KO Plasmid (h) | sc-405886 | 20 µg | $397.00 |
Overview
SENP7 (sentrin-specific protease 7) is a SUMO-specific isopeptidase that removes SUMO2/3 modifications from target proteins, thereby regulating SUMOylation dynamics and protein function. It has been linked to chromatin-associated processes, including heterochromatin maintenance and the DNA damage response, where SUMO-dependent signaling helps coordinate repair factor recruitment and genome stability. Through these activities, SENP7 influences cell cycle progression and transcriptional programs that are sensitive to chromatin state. Dysregulated SUMO protease activity, including altered SENP7 function, is relevant to studies of oncogenic stress, replication-associated damage, and other contexts where aberrant chromatin regulation contributes to disease phenotypes.
SENP7 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SENP7 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SENP7 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SENP7 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SENP7 protein expression.
This CRISPR knockout system enables efficient generation of SENP7-deficient cell models for investigation of SENP7 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SENP7 exon(s) critical for SENP7 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SENP7 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SENP7 CRISPR/Cas9 KO Plasmid (h) and SENP7 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SENP7 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SENP7 HDR Plasmid (h) and SENP7 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SENP7 homology arms to support homology-directed repair at defined SENP7 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.