SEMA7A Double Nickase Plasmid (h): sc-403873-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SEMA7A Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SEMA7A Double Nickase Plasmid (h) and SEMA7A Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SEMA7A. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SEMA7A Antibody (C-6): sc-374432
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SEMA7A Double Nickase Plasmid (h) | sc-403873-NIC | 20 µg | $410.00 | |||
SEMA7A Double Nickase Plasmid (h2) | sc-403873-NIC-2 | 20 µg | $410.00 |
SEMA7A (semaphorin 7A/CD108) is a glycosylphosphatidylinositol-anchored guidance and immunoregulatory protein that mediates cell–cell and cell–matrix interactions. It signals through receptors including integrins and plexin pathways to modulate cytoskeletal remodeling, adhesion, migration, and axon outgrowth, and it can influence inflammatory activation and tissue remodeling programs. In human cells, SEMA7A has been implicated in regulation of immune cell trafficking and fibrotic responses, linking it to mechanisms relevant to chronic inflammation, neurobiology, and microenvironmental remodeling. Dysregulated SEMA7A expression or signaling has been associated with pathological changes in fibrosis and tumor-associated immune and stromal dynamics, supporting its use as a target in mechanistic studies.
SEMA7A Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SEMA7A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SEMA7A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SEMA7A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SEMA7A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.