Seipin Double Nickase Plasmid (h): sc-406865-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Seipin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Seipin Double Nickase Plasmid (h) and Seipin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BSCL2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Seipin Double Nickase Plasmid (h) | sc-406865-NIC | 20 µg | $410.00 | |||
Seipin Double Nickase Plasmid (h2) | sc-406865-NIC-2 | 20 µg | $410.00 |
Human BSCL2 encodes seipin, an endoplasmic reticulum membrane protein that organizes lipid droplet biogenesis and promotes proper triacylglycerol storage through coordination of ER–lipid droplet contact sites. Seipin influences adipocyte differentiation and lipid homeostasis pathways, linking neutral lipid synthesis, phospholipid remodeling, and organelle membrane dynamics. Disruption of BSCL2 perturbs lipid droplet morphology and cellular energy balance, with established relevance to congenital generalized lipodystrophy and seipin-associated neurodegenerative phenotypes, including distal hereditary motor neuropathy and related disorders. These connections make BSCL2 a useful node for studying lipid metabolism, proteostasis stress responses, and cell type–specific vulnerability.
Seipin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BSCL2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BSCL2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BSCL2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BSCL2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.