SEC16L Double Nickase Plasmid (h): sc-411387-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SEC16L Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SEC16L Double Nickase Plasmid (h) and SEC16L Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SEC16A. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SEC16L Double Nickase Plasmid (h) | sc-411387-NIC | 20 µg | $410.00 | |||
SEC16L Double Nickase Plasmid (h2) | sc-411387-NIC-2 | 20 µg | $410.00 |
SEC16A encodes SEC16L, a peripheral membrane scaffold that organizes endoplasmic reticulum (ER) exit sites and coordinates COPII coat assembly during ER-to-Golgi transport. By regulating the recruitment and turnover of COPII components, SEC16L helps control secretory flux, membrane trafficking, and organelle homeostasis, with downstream effects on proteostasis and cellular stress adaptation. Perturbation of early secretory pathway function can influence signaling receptor trafficking and metabolic regulation, and SEC16A/SEC16L dysregulation has been investigated in contexts where ER stress, secretion, and membrane dynamics contribute to disease-relevant phenotypes. As a result, SEC16L is a useful target for studying how ER export interfaces with autophagy, unfolded protein response signaling, and trafficking-dependent control of cell state.
SEC16L Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SEC16A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SEC16A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SEC16A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SEC16A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.