SCUBE2 Antibody (G-4): sc-398607
- SCUBE2 Antibody (G-4) is a mouse monoclonal IgG1 κ SCUBE2 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 570-639 mapping within an internal region of SCUBE2 of human origin
- SCUBE2 Antibody (G-4) is recommended for detection of SCUBE2 of human origin by WB, IP, IF and ELISA
- Anti-SCUBE2 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for SCUBE2 Antibody (G-4) for WB applications. These reagents are now offered in bundles with SCUBE2 Antibody (G-4) (see ordering information below).
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SCUBE2 Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects SCUBE2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SCUBE2 Antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SCUBE2, also known as signal peptide, CUB domain, EGF-like 2, CEGP1, Cegb1, or Cegf1, is a 999 amino acid protein widely expressed across adult tissues and belongs to the evolutionarily conserved SCUBE protein family. SCUBE2 features a CUB domain and nine EGF-like domains, and when overexpressed, acts as a secreted surface-anchored glycoprotein, playing a crucial role as a novel component of the Hedgehog signaling pathway. The Hedgehog signal is essential for the induction of ventral neuronal and muscle cell types during vertebrate development, highlighting SCUBE2′s importance in developmental biology. SCUBE2 has been implicated in breast cancer progression, suggesting potential use as a prognostic marker. SCUBE2 forms homo-oligomers and hetero-oligomers with other SCUBE family members, specifically SCUBE1 and SCUBE3. A gene located on human chromosome 11, which contains over 1,400 genes and represents nearly 4% of the human genome, encodes SCUBE2, expressing three isoforms through alternative splicing events.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SCUBE2 Antibody (G-4) References:
- Expression of a novel mammalian epidermal growth factor-related gene during mouse neural development. | Grimmond, S., et al. 2001. Mech Dev. 102: 209-11. PMID: 11287194
- Identification of a novel family of cell-surface proteins expressed in human vascular endothelium. | Yang, RB., et al. 2002. J Biol Chem. 277: 46364-73. PMID: 12270931
- The you gene encodes an EGF-CUB protein essential for Hedgehog signaling in zebrafish. | Woods, IG. and Talbot, WS. 2005. PLoS Biol. 3: e66. PMID: 15660164
- The zebrafish-secreted matrix protein you/scube2 is implicated in long-range regulation of hedgehog signaling. | Kawakami, A., et al. 2005. Curr Biol. 15: 480-8. PMID: 15753045
- Scube2 mediates Hedgehog signalling in the zebrafish embryo. | Hollway, GE., et al. 2006. Dev Biol. 294: 104-18. PMID: 16626681
- SCUBE2 suppresses breast tumor cell proliferation and confers a favorable prognosis in invasive breast cancer. | Cheng, CJ., et al. 2009. Cancer Res. 69: 3634-41. PMID: 19369267
- Isolation and characterization of a secreted, cell-surface glycoprotein SCUBE2 from humans. | Tsai, MT., et al. 2009. Biochem J. 422: 119-28. PMID: 19480626
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SCUBE2 Antibody (G-4) | sc-398607 | 200 µg/ml | $322.00 | |||
SCUBE2 Antibody (G-4): m-IgG Fc BP-HRP Bundle | sc-530870 | 200 µg Ab; 10 µg BP | $361.00 | |||
SCUBE2 Antibody (G-4): m-IgGκ BP-HRP Bundle | sc-524419 | 200 µg Ab, 40 µg BP | $361.00 | |||
SCUBE2 Antibody (G-4): m-IgG1 BP-HRP Bundle | sc-544290 | 200 µg Ab; 20 µg BP | $361.00 | |||
SCUBE2 Antibody (G-4) AC | sc-398607 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SCUBE2 Antibody (G-4) HRP | sc-398607 HRP | 200 µg/ml | $322.00 | |||
SCUBE2 Antibody (G-4) FITC | sc-398607 FITC | 200 µg/ml | $336.00 | |||
SCUBE2 Antibody (G-4) PE | sc-398607 PE | 200 µg/ml | $349.00 | |||
SCUBE2 Antibody (G-4) Alexa Fluor® 488 | sc-398607 AF488 | 200 µg/ml | $364.00 | |||
SCUBE2 Antibody (G-4) Alexa Fluor® 546 | sc-398607 AF546 | 200 µg/ml | $364.00 | |||
SCUBE2 Antibody (G-4) Alexa Fluor® 594 | sc-398607 AF594 | 200 µg/ml | $364.00 | |||
SCUBE2 Antibody (G-4) Alexa Fluor® 647 | sc-398607 AF647 | 200 µg/ml | $364.00 | |||
SCUBE2 Antibody (G-4) Alexa Fluor® 680 | sc-398607 AF680 | 200 µg/ml | $364.00 | |||
SCUBE2 Antibody (G-4) Alexa Fluor® 790 | sc-398607 AF790 | 200 µg/ml | $364.00 |