SCGF Double Nickase Plasmid (h): sc-411000-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SCGF Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SCGF Double Nickase Plasmid (h) and SCGF Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CLEC11A. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SCGF Double Nickase Plasmid (h) | sc-411000-NIC | 20 µg | $410.00 | |||
SCGF Double Nickase Plasmid (h2) | sc-411000-NIC-2 | 20 µg | $410.00 |
CLEC11A encodes stem cell growth factor (SCGF), a secreted C-type lectin family cytokine implicated in hematopoietic stem and progenitor cell maintenance and stromal–hematopoietic niche signaling. SCGF supports progenitor proliferation and survival programs and is commonly studied in the context of cytokine-regulated differentiation, inflammatory signaling crosstalk, and bone marrow microenvironment biology. Altered CLEC11A/SCGF expression has been associated with dysregulated hematopoiesis and tumor–stroma interactions, making it relevant to research on myeloid lineage bias, marrow failure phenotypes, and cancer-associated microenvironment remodeling. These attributes position CLEC11A as a useful target for dissecting extracellular cues that influence stem cell fate decisions and immune cell development.
SCGF Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CLEC11A locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CLEC11A. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CLEC11A function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CLEC11A-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.