Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SCD1 Double Nickase Plasmid (m): sc-422809-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SCD1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SCD1 Double Nickase Plasmid (m) and SCD1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Scd1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SCD1 Antibody (E-8): sc-515844
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SCD1 Double Nickase Plasmid (m) | sc-422809-NIC | 20 µg | $410.00 |
Mouse Scd1 encodes stearoyl-CoA desaturase 1 (SCD1), an endoplasmic reticulum–localized enzyme that introduces a cis double bond into saturated fatty acyl-CoAs to generate monounsaturated fatty acids such as palmitoleate and oleate. By controlling the saturated/monounsaturated lipid balance, SCD1 regulates de novo lipogenesis, membrane phospholipid composition, triglyceride and cholesteryl ester synthesis, and lipid droplet biogenesis, with downstream effects on ER stress responses and metabolic signaling. Scd1 activity intersects with nutrient-sensing transcriptional programs including SREBP1 and PPAR pathways, shaping cellular energy storage and redox homeostasis. Altered SCD1 expression or activity has been linked to metabolic phenotypes involving hepatic steatosis and obesity-related dysregulation, and it is frequently studied in contexts of lipid remodeling in proliferative and inflammatory states.
SCD1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Scd1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Scd1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Scd1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Scd1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.