SBP Tag Antibody (SB19-C4): sc-101595

SBP Tag Antibody (SB19-C4) is a mouse monoclonal IgG1 kappa light chain antibody that detects the SBP Tag protein in various species through applications such as western blotting (WB) and immunofluorescence (IF). Anti-SBP Tag antibody (SB19-C4) is available in both non-conjugated and multiple conjugated forms, including agarose, phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatility for different experimental needs. The SBP Tag, a 38 amino acid sequence, is specifically designed to bind to streptavidin, a tetrameric protein known for its exceptionally strong affinity for biotin, with a dissociation constant (Kd) of 10^-14 mol/L. This robust interaction is crucial for numerous applications in molecular biology, as SBP Tag monoclonal antibody (SB19-C4) allows for precise detection and purification of recombinant proteins. The SBP Tag′s ability to form stable complexes with streptavidin enables researchers to effectively combine various biomolecules, facilitating the development of complex assays and enhancing detection sensitivity. Additionally, the SBP Tag is instrumental in immunochemistry purification, making SBP Tag (SB19-C4) antibody a vital component in diagnostic and laboratory kits. Using SBP Tag (SB19-C4) monoclonal antibody allows researchers to leverage this unique protein interaction to advance studies in protein characterization and analysis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SBP Tag Antibody (SB19-C4) References:

1. One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag.  |  Keefe, AD., et al. 2001. Protein Expr Purif. 23: 440-6. PMID: 11722181
2. Ligand exchange between proteins. Exchange of biotin and biotin derivatives between avidin and streptavidin.  |  Pazy, Y., et al. 2002. J Biol Chem. 277: 30892-900. PMID: 12055191
3. Genetic engineering of streptavidin-binding peptide tagged single-chain variable fragment antibody to Venezuelan equine encephalitis virus.  |  Hu, WG., et al. 2002. Hybrid Hybridomics. 21: 415-20. PMID: 12573105
4. The Nano-tag, a streptavidin-binding peptide for the purification and detection of recombinant proteins.  |  Lamla, T. and Erdmann, VA. 2004. Protein Expr Purif. 33: 39-47. PMID: 14680960
5. A favorable solubility partner for the recombinant expression of streptavidin.  |  Sørensen, HP., et al. 2003. Protein Expr Purif. 32: 252-9. PMID: 14965771
6. Cooperative hydrogen bond interactions in the streptavidin-biotin system.  |  Hyre, DE., et al. 2006. Protein Sci. 15: 459-67. PMID: 16452627
7. A monovalent streptavidin with a single femtomolar biotin binding site.  |  Howarth, M., et al. 2006. Nat Methods. 3: 267-73. PMID: 16554831
8. The high-resolution structure of (+)-epi-biotin bound to streptavidin.  |  Le Trong, I., et al. 2006. Acta Crystallogr D Biol Crystallogr. 62: 576-81. PMID: 16699183
9. Analysis of protein-DNA binding by streptavidin-agarose pulldown.  |  Wu, KK. 2006. Methods Mol Biol. 338: 281-90. PMID: 16888365
10. Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex.  |  Kim, JH., et al. 2010. BMC Biochem. 11: 50. PMID: 21194474
11. Structure-guided design of an engineered streptavidin with reusability to purify streptavidin-binding peptide tagged proteins or biotinylated proteins.  |  Wu, SC. and Wong, SL. 2013. PLoS One. 8: e69530. PMID: 23874971
12. Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag.  |  Yang, L. and Veraksa, A. 2017. Methods Mol Biol. 1487: 113-126. PMID: 27924562

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SBP Tag Antibody (SB19-C4) has 58 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SBP Tag Antibody (SB19-C4) Specifications

Alternate Product Name: SBP Tag

Product Clone Name: SB19-C4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: SBP Tag

Epitope Species:

Product Reactivity/Detection: SBP tag

Species Reactivity/Detection:

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 58 citations in various scientific publications

Available Conjugates/Forms for SBP Tag Antibody (SB19-C4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, PE: Phycoerythrin