Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SAP 97 Double Nickase Plasmid (h): sc-400865-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SAP 97 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SAP 97 Double Nickase Plasmid (h) and SAP 97 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DLG1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SAP 97 Antibody (2D11): sc-9961
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SAP 97 Double Nickase Plasmid (h) | sc-400865-NIC | 20 µg | $410.00 | |||
SAP 97 Double Nickase Plasmid (h2) | sc-400865-NIC-2 | 20 µg | $410.00 |
DLG1 encodes synapse-associated protein 97 (SAP97), a membrane-associated guanylate kinase (MAGUK) scaffold that organizes multiprotein complexes at cell–cell junctions and neuronal synapses. SAP97 coordinates trafficking and stabilization of ionotropic glutamate receptors and other channels, linking them to cytoskeletal and signaling components to shape synaptic strength and polarity. Through PDZ, SH3, and GK domain–mediated interactions, SAP97 integrates signaling modules involved in adhesion, excitability, and junctional remodeling. Altered DLG1/SAP97 expression or localization has been studied in contexts of neurodevelopmental and neuropsychiatric phenotypes, as well as epithelial polarity disruption and tumor-associated changes in cell adhesion pathways.
SAP 97 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DLG1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DLG1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DLG1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DLG1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.