Molecular structure of SAG, CAS Number: 912545-86-9
SAG (CAS 912545-86-9)
See product citations (27)
Alternate Names:
SAG is also known as Smoothened Agonist.
Application:
SAG is a potent agonist of Hedgehog (Hh) signalling, inhibiting at concentrations higher than 1 µM.
CAS Number:
912545-86-9
Purity:
≥95%
Molecular Weight:
490.06
Molecular Formula:
C28H28ClN3OS
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
SAG is a potent agonist of Hedgehog (Hh) signalling. It operates by activating the Smoothened (Smo) protein function. SAG directly binds to Smo and can block Smo inhibition by cyclopamine. This binding of SAG alters the Smo conformation, causing an accumulation in cilia and activation of gene transcription. The conversion of human induced pluripotent stem fcells to the neural lineage is improved by higher concentrations of SAG (0.4 mM).
SAG (CAS 912545-86-9) References
- Smoothened activates Galphai-mediated signaling in frog melanophores. | DeCamp, DL., et al. 2000. J Biol Chem. 275: 26322-7. PMID: 10835429
- Small molecule modulation of Smoothened activity. | Chen, JK., et al. 2002. Proc Natl Acad Sci U S A. 99: 14071-6. PMID: 12391318
- Small-molecule modulators of Hedgehog signaling: identification and characterization of Smoothened agonists and antagonists. | Frank-Kamenetsky, M., et al. 2002. J Biol. 1: 10. PMID: 12437772
- In vivo analysis of quiescent adult neural stem cells responding to Sonic hedgehog. | Ahn, S. and Joyner, AL. 2005. Nature. 437: 894-7. PMID: 16208373
- Signaling from Smo to Ci/Gli: conservation and divergence of Hedgehog pathways from Drosophila to vertebrates. | Huangfu, D. and Anderson, KV. 2006. Development. 133: 3-14. PMID: 16339192
- Increase of proliferating oligodendroglial progenitors in the adult mouse brain upon Sonic hedgehog delivery in the lateral ventricle. | Loulier, K., et al. 2006. J Neurochem. 98: 530-42. PMID: 16805844
- Hedgehog signal transduction by Smoothened: pharmacologic evidence for a 2-step activation process. | Rohatgi, R., et al. 2009. Proc Natl Acad Sci U S A. 106: 3196-201. PMID: 19218434
- Converse conformational control of smoothened activity by structurally related small molecules. | Yang, H., et al. 2009. J Biol Chem. 284: 20876-84. PMID: 19366682
- A small-molecule smoothened agonist prevents glucocorticoid-induced neonatal cerebellar injury. | Heine, VM., et al. 2011. Sci Transl Med. 3: 105ra104. PMID: 22013124
- Small molecules greatly improve conversion of human-induced pluripotent stem cells to the neuronal lineage. | Mak, SK., et al. 2012. Stem Cells Int. 2012: 140427. PMID: 22567022
- Smoothened-dependent and -independent pathways in mammalian noncanonical Hedgehog signaling. | Faria, AVS., et al. 2019. J Biol Chem. 294: 9787-9798. PMID: 30992365
- Activation of sonic hedgehog signaling by a Smoothened agonist restores congenital defects in mouse models of endocrine-cerebro-osteodysplasia syndrome. | Shin, JO., et al. 2019. EBioMedicine. 49: 305-317. PMID: 31662288
- The smoothened agonist SAG reduces mitochondrial dysfunction and neurotoxicity of frataxin-deficient astrocytes. | Vicente-Acosta, A., et al. 2022. J Neuroinflammation. 19: 93. PMID: 35413853
- Bone-Targeting Exosome Mimetics Engineered by Bioorthogonal Surface Functionalization for Bone Tissue Engineering. | Lee, CS., et al. 2023. Nano Lett.. PMID: 36762874
Inhibitor of:
DCHS2, EG435927, FEZF1, HESX1, IL-3Rβ, Olfr1043, Rsph10b2, SEMA3E, and UBE1Y1.Activator of:
ACAT-1, ASCL5, Barx2, BBS7, cubitus interruptus, DISP2, Distal-less, DZIP1, DZIP1L, EVC2, FBXO22, FOXN4, GLI-1, GLI-3, GLI-4, glypican-6, Grcc10, hedgehog, HES7, HH, HIP3, HMX1, HoxA1, HRT1_Hrt1, ITM2A, Jagged, KIAA1370, KIF7, Ksr-1, MAML2, MESP2, MIA2, Nkx-2.2, OLIG2, Paraxis, patched 2, Phox2b, PHYHIP, PTCHD1, PTCHD3, Radical Fringe, RasGRP3, robo2, SCR, SCUBE3, Six3, Smo, Smurf1, STK36, Su(fu), TBX5, TMEM46, TSHZ1, and ZEB2.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SAG, 1 mg | sc-212905 | 1 mg | $166.00 | |||
SAG, 5 mg | sc-212905A | 5 mg | $421.00 |