(S)-1-(p-Bromoacetamidobenzyl)ethylenediaminetetraacetic Acid (CAS 81677-64-7)

(S)-1-(p-Bromoacetamidobenzyl)ethylenediaminetetraacetic Acid is a specialized chelator used predominantly in the study of biochemical interactions, particularly in the context of calcium ion management within biological systems. This compound combines a bromoacetamidobenzyl group with the well-known chelating structure of ethylenediaminetetraacetic acid (EDTA), allowing it to bind divalent metal ions with high specificity and efficiency. The unique aspect of this molecule lies in its bromoacetamidobenzyl moiety, which provides a reactive site for covalent bonding with thiol groups on cysteine residues of proteins. This feature is exploited in research to tag or immobilize proteins on various surfaces or within gels, facilitating the analysis of protein interactions and functions. The calcium chelation ability of this compound is particularly valuable in studies where precise control over calcium concentrations is necessary to understand calcium′s role in cellular signaling, enzyme activation, and muscle contraction. By binding calcium ions, (S)-1-(p-Bromoacetamidobenzyl)ethylenediaminetetraacetic Acid helps researchers manipulate and monitor calcium-dependent processes in vitro, providing insights into how cells utilize calcium signaling in physiological and pathological conditions. Its application extends beyond simple chelation; it is also used in the development of new biochemical techniques for studying metal-protein interactions, enhancing the depth and scope of molecular biology research.