RUNX2 Antibody (27-K): sc-101145

RUNX2 Antibody (27-K) is a mouse monoclonal IgG2b antibody that detects RUNX2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-RUNX2 antibody (27-K) is available as the non-conjugated form. RUNX2, a member of the Runt-related transcription factor family, plays a critical role in skeletal development and mineralization by regulating osteoblast differentiation from mesenchymal stem cells. RUNX2 (27-K) monoclonal antibody targets a transcription factor that′s pivotal for bone formation, chondrocyte hypertrophy, and endothelial cell migration - essential processes in skeletal system development. Proper functioning of RUNX2 is vital, as dysregulation can lead to severe skeletal disorders, including cleidocranial dysplasia syndrome, characterized by abnormal bone development and dental anomalies. RUNX2 interacts with various proteins, including osterix and β-catenin, which are crucial for bone formation and remodeling. Understanding these interactions and regulatory mechanisms helps researchers explore potential therapeutic strategies to enhance bone regeneration and treat related disorders.

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RUNX2 Antibody (27-K) References:

1. Interaction and functional cooperation of PEBP2/CBF with Smads. Synergistic induction of the immunoglobulin germline Calpha promoter.  |  Hanai, J., et al. 1999. J Biol Chem. 274: 31577-82. PMID: 10531362
2. Role of Runx proteins in chondrogenesis.  |  Yoshida, CA. and Komori, T. 2005. Crit Rev Eukaryot Gene Expr. 15: 243-54. PMID: 16390320
3. Transcriptional co-repressors of Runx2.  |  Westendorf, JJ. 2006. J Cell Biochem. 98: 54-64. PMID: 16440320
4. Cleidocranial dysplasia with severe parietal bone dysplasia: C-terminal RUNX2 mutations.  |  Cunningham, ML., et al. 2006. Birth Defects Res A Clin Mol Teratol. 76: 78-85. PMID: 16463420
5. Purification of a mouse nuclear factor that binds to both the A and B cores of the polyomavirus enhancer.  |  Kamachi, Y., et al. 1990. J Virol. 64: 4808-19. PMID: 2168969
6. Regulation of Proliferation, Differentiation and Functions of Osteoblasts by Runx2.  |  Komori, T. 2019. Int J Mol Sci. 20: PMID: 30987410
7. Whole Aspect of Runx2 Functions in Skeletal Development.  |  Komori, T. 2022. Int J Mol Sci. 23: PMID: 35628587
8. Runx2 activates hepatic stellate cells to promote liver fibrosis via transcriptionally regulating Itgav expression.  |  Zhong, L., et al. 2023. Clin Transl Med. 13: e1316. PMID: 37403784
9. PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila runt gene and the human AML1 gene.  |  Ogawa, E., et al. 1993. Proc Natl Acad Sci U S A. 90: 6859-63. PMID: 8341710
10. Molecular cloning and characterization of PEBP2 beta, the heterodimeric partner of a novel Drosophila runt-related DNA binding protein PEBP2 alpha.  |  Ogawa, E., et al. 1993. Virology. 194: 314-31. PMID: 8386878
11. Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation.  |  Ducy, P., et al. 1997. Cell. 89: 747-54. PMID: 9182762
12. Mutations involving the transcription factor CBFA1 cause cleidocranial dysplasia.  |  Mundlos, S., et al. 1997. Cell. 89: 773-9. PMID: 9182765

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RUNX2 Antibody (27-K) has 105 citations in a variety of scientific publications.

Primary antibodies like Anti-RUNX2 Antibody (27-K) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RUNX2 Antibody (27-K) Specifications

Alternate Product Name: RUNX2

Product Clone Name: 27-K

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids: 251-351

Epitope Target: RUNX2

Epitope Species: human

Product Reactivity/Detection: RUNX2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 50 µg IgG_2b in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 105 citations in various scientific publications

Available Conjugates/Forms for RUNX2 Antibody (27-K): N/A

The preferred RUNX2 antibody is RUNX2 Antibody (F-2): sc-390351.