Running Buffer, 10X is a Tris-Glycine buffer used for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. It is used as both the anode and cathode buffer. Running Buffer, 10X is supplied as 1L of 10X concentrate that can be diluted to a 1X solution containing 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3. Researchers may check and adjust the final pH using a sensitive pH meter.
1. Laemmli, U.K. 1970. Nature. 227: 680-685. PMID: 5432063
2. Roder, K., et al. 2001. Biotechnol. Appl. Biochem. 33: 209-214. PMID: 11389675
See how others have used Running Buffer, 10X. Click on the entry to view the PubMed entry .
PMID: # 22708801 Kjartansdóttir, KR. et al. 2012. Systems biology in reproductive medicine. 58: 330-8.
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