RRP9 Antibody (C-7): sc-515661

RRP9 Antibody (C-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects RRP9 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RRP9 (C-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RRP9 protein, also known as ribosomal RNA processing 9, small subunit processome component, RNU3IP2, or U355K, is a crucial 475 amino acid nucleolar protein that plays a significant role in the modification and processing of precursor ribosomal RNA (rRNA). Specifically, RRP9 is a component of a small nucleolar ribonucleoprotein particle (snoRNP) and is essential for interacting with the U3 snoRNA complex, binding to a fragment known as 3UBC, which contains a box B/C motif. The functionality of RRP9 in pre-rRNA processing is contingent upon the presence of the snRNP protein NHPX, which binds to the B/C motif, and a conserved tertiary structure that flanks this motif. The interaction between RRP9 and 3UBC is vital for the proper processing of rRNA, underscoring RRP9′s importance in ribosome biogenesis. Additionally, RRP9 contains seven WD repeats that are critical for nucleolar localization and U3 snoRNA binding, highlighting RRP9′s structural significance in cellular processes.

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RRP9 Antibody (C-7) References:

1. Interaction of the U3-55k protein with U3 snoRNA is mediated by the box B/C motif of U3 and the WD repeats of U3-55k.  |  Lukowiak, AA., et al. 2000. Nucleic Acids Res. 28: 3462-71. PMID: 10982864
2. Yeast Rrp9p is an evolutionarily conserved U3 snoRNP protein essential for early pre-rRNA processing cleavages and requires box C for its association.  |  Venema, J., et al. 2000. RNA. 6: 1660-71. PMID: 11105764
3. 90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors.  |  Grandi, P., et al. 2002. Mol Cell. 10: 105-15. PMID: 12150911
4. The hU3-55K protein requires 15.5K binding to the box B/C motif as well as flanking RNA elements for its association with the U3 small nucleolar RNA in Vitro.  |  Granneman, S., et al. 2002. J Biol Chem. 277: 48490-500. PMID: 12381732
5. A structural, phylogenetic, and functional study of 15.5-kD/Snu13 protein binding on U3 small nucleolar RNA.  |  Marmier-Gourrier, N., et al. 2003. RNA. 9: 821-38. PMID: 12810916
6. Assembly and maturation of the U3 snoRNP in the nucleoplasm in a large dynamic multiprotein complex.  |  Watkins, NJ., et al. 2004. Mol Cell. 16: 789-98. PMID: 15574333
7. Nucleolar proteome dynamics.  |  Andersen, JS., et al. 2005. Nature. 433: 77-83. PMID: 15635413
8. cDNA cloning and characterization of the human U3 small nucleolar ribonucleoprotein complex-associated 55-kilodalton protein.  |  Pluk, H., et al. 1998. Mol Cell Biol. 18: 488-98. PMID: 9418896

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RRP9 Antibody (C-7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-RRP9 Antibody (C-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RRP9 Antibody (C-7) Specifications

Alternate Product Name: RRP9

Product Clone Name: C-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 271-370

Epitope Target: RRP9

Epitope Species: human

Product Reactivity/Detection: RRP9

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for RRP9 Antibody (C-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin