RPA 70 kDa subunit Antibody (MA70-2): sc-53497

RPA 70 kDa subunit Antibody (MA70-2) is a mouse monoclonal IgG1 antibody that detects RPA 70 kDa subunit in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-RPA 70 kDa subunit antibody (MA70-2) targets the highly conserved eukaryotic single-stranded DNA-binding protein, which plays a crucial role in DNA replication, recombination, and repair. RPA 70 kDa subunit (MA70-2) antibody recognizes a protein essential for maintaining genomic stability, as RPA 70 kDa subunit binds to single-stranded DNA during the early stages of DNA damage response, facilitating the recruitment of other repair factors. RPA 70 kDa subunit forms a stable complex with single-stranded DNA, which is vital for the proper functioning of nucleotide excision repair pathways. RPA 70 kDa subunit′s structure includes a DNA binding domain composed of two homologous subdomains, which enhances affinity for ssDNA. A conserved four cysteine-type zinc-finger motif within RPA 70 kDa subunit mediates the transition from a transient to a stable RPA-ssDNA complex in a redox-dependent manner, underscoring RPA 70 kDa subunit′s role in cellular responses to DNA damage. Through interactions with various cellular and viral proteins, RPA 70 kDa subunit maintains telomeres and overall genome integrity, making RPA 70 kDa subunit (MA70-2) monoclonal antibody an invaluable tool for researchers studying DNA repair mechanisms and related cellular processes.

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RPA 70 kDa subunit Antibody (MA70-2) References:

1. Replication protein A interactions with DNA. 1. Functions of the DNA-binding and zinc-finger domains of the 70-kDa subunit.  |  Walther, AP., et al. 1999. Biochemistry. 38: 3963-73. PMID: 10194308
2. The weak interdomain coupling observed in the 70 kDa subunit of human replication protein A is unaffected by ssDNA binding.  |  Daughdrill, GW., et al. 2001. Nucleic Acids Res. 29: 3270-6. PMID: 11470885
3. The phosphorylation domain of the 32-kDa subunit of replication protein A (RPA) modulates RPA-DNA interactions. Evidence for an intersubunit interaction.  |  Binz, SK., et al. 2003. J Biol Chem. 278: 35584-91. PMID: 12819197
4. Chemical shift changes provide evidence for overlapping single-stranded DNA- and XPA-binding sites on the 70 kDa subunit of human replication protein A.  |  Daughdrill, GW., et al. 2003. Nucleic Acids Res. 31: 4176-83. PMID: 12853635
5. 1H, 13C and 15N assignments of single-stranded DNA binding domains from the 70 kDa subunit of human replication protein A.  |  Bhattacharya, S., et al. 2004. J Biomol NMR. 28: 195-6. PMID: 14755166
6. Characterization of a cDNA encoding the 70-kDa single-stranded DNA-binding subunit of human replication protein A and the role of the protein in DNA replication.  |  Erdile, LF., et al. 1991. J Biol Chem. 266: 12090-8. PMID: 2050703
7. Discovery of Protein-Protein Interaction Inhibitors of Replication Protein A.  |  Patrone, JD., et al. 2013. ACS Med Chem Lett. 4: 601-605. PMID: 23914285
8. DNA unwinding by replication protein A is a property of the 70 kDa subunit and is facilitated by phosphorylation of the 32 kDa subunit.  |  Georgaki, A. and Hübscher, U. 1993. Nucleic Acids Res. 21: 3659-65. PMID: 8396234
9. Functional domains of the 70-kilodalton subunit of human replication protein A.  |  Gomes, XV. and Wold, MS. 1996. Biochemistry. 35: 10558-68. PMID: 8756712
10. Replication protein A. Characterization and crystallization of the DNA binding domain.  |  Pfuetzner, RA., et al. 1997. J Biol Chem. 272: 430-4. PMID: 8995279

The preferred RPA 70 kDa subunit antibody is RPA 70 kDa subunit Antibody (B-6): sc-28304.