Anti-RPA 32 kDa subunit Antibody (B-4) recommended for detection of RPA 32 kDa subunit of human origin by WB, IP, IF and ELISA

Anti-RPA 32 kDa subunit Antibody (B-4): sc-271578

Anti-RPA 32 kDa subunit Antibody (B-4) is rated 5.0 out of 5 by 2.
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Datasheets

    • RPA 32 kDa subunit Antibody (B-4) is a mouse monoclonal IgG2b κ, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 155-254 mapping within an internal region of RPA 32 kDa subunit of human origin
    • recommended for detection of RPA 32 kDa subunit of human origin by WB, IP, IF and ELISA
    • TransCruz reagent for ChIP application (sc-271578 X, 200 µg/0.1 ml)
    • See RPA 32 kDa subunit (9H8): sc-56770 for RPA 32 kDa subunit antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • m-IgG2b BP-HRP is the preferred secondary detection reagent for RPA 32 kDa subunit Antibody (B-4) for WB applications. This reagent is now offered in a bundle with RPA 32 kDa subunit Antibody (B-4) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with RPA 32 kDa subunit (B-4): sc-271578 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Produced great immunofluorescence nuclear Produced great immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-20
Rated 5 out of 5 by from Good Western Blot data of RPA 32 kDa subunit Good Western Blot data of RPA 32 kDa subunit expression in HeLa, H69AR and T-47D whole cell lysates. -SCBT QC
Date published: 2014-09-29
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