Date published: 2026-2-15

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RPA 32 kDa subunit Antibody (B-4): sc-271578

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Datasheets
  • RPA 32 kDa subunit Antibody (B-4) is a mouse monoclonal IgG2b κ, cited in 3 publications, provided at 200 µg/ml
  • raised against amino acids 155-254 mapping within an internal region of RPA 32 kDa subunit of human origin
  • recommended for detection of RPA 32 kDa subunit of human origin by WB, IP, IF and ELISA
  • TransCruz reagent for ChIP application (sc-271578 X, 200 µg/0.1 ml)
  • See RPA 32 kDa subunit (9H8): sc-56770 for RPA 32 kDa subunit antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG2b BP-HRP is the preferred secondary detection reagent for RPA 32 kDa subunit Antibody (B-4) for WB applications. This reagent is now offered in a bundle with RPA 32 kDa subunit Antibody (B-4) (see ordering information below).
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RPA 32 kDa subunit Antibody (B-4) is a mouse monoclonal IgG2b antibody that detects RPA 32 kDa subunit in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RPA 32 kDa subunit is a critical component of the heterotrimeric replication protein A complex, which plays a vital role in DNA replication, recombination, and repair processes. RPA 32 kDa subunit is primarily located in the nucleus, where RPA 32 kDa subunit binds to single-stranded DNA, facilitating the stabilization of DNA during replication and repair. RPA 32′s ability to interact with various DNA repair enzymes, such as UNG2, XPA, and Rad52, underscores RPA 32′s importance in maintaining genomic integrity. Additionally, RPA 32 kDa subunit is involved in telomere maintenance, which is crucial for cellular aging and stability. RPA 32′s interaction with the SH2 domain of Stat3 highlights RPA 32′s role in signaling pathways, where overexpression can enhance growth factor-stimulated tyrosine phosphorylation and transcriptional activity, further emphasizing RPA 32′s significance in cellular processes. Anti-RPA 32 kDa subunit antibody (B-4) is an essential tool for researchers studying DNA repair mechanisms and related signaling pathways in human cells.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

RPA 32 kDa subunit Antibody (B-4)

sc-271578
200 µg/ml
$322.00

RPA 32 kDa subunit Antibody (B-4): m-IgG2b BP-HRP Bundle

sc-548542
200 µg Ab; 10 µg BP
$361.00

RPA 32 kDa subunit Antibody (B-4) X

sc-271578 X
200 µg/0.1 ml
$322.00

What is the recommended fixation method for immunofluorescence staining with RPA 32 kDa subunit (B-4): sc-271578 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Produced great immunofluorescence nuclearProduced great immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-02-20
Rated 5 out of 5 by from Good Western Blot data of RPA 32 kDa subunitGood Western Blot data of RPA 32 kDa subunit expression in HeLa, H69AR and T-47D whole cell lysates. -SCBT QC
Date published: 2014-09-29
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RPA 32 kDa subunit Antibody (B-4) is rated 5.0 out of 5 by 2.
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