Rotavirus capsid Antibody (2B4): sc-101363

Rotavirus capsid Antibody (2B4) is a mouse monoclonal IgG2b kappa light chain antibody that detects the inner capsid protein VP6 of Rotavirus origin by western blotting (WB), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Rotavirus capsid antibody (2B4) is available in a non-conjugated form, providing researchers with a versatile tool for studying this significant pathogen. Rotaviruses, belonging to the Reoviridae family, are double-stranded RNA viruses and are among the leading causes of gastroenteritis in infants and young children, with Rotavirus A being the most prevalent strain. The importance of VP6 protein lies in its role as a structural component of the virus, forming the inner layer of the capsid that protects the viral RNA genome. VP6 protein is crucial for viral stability and integrity, facilitating host cell infection and immune response evasion. VP6 protein serves as a target for neutralizing antibodies, making this protein a key focus for vaccine development. Understanding VP6 function and host cell machinery interactions can provide insights into rotavirus pathogenesis mechanisms and inform prevention and treatment strategies.

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Rotavirus capsid Antibody (2B4) References:

1. Rotavirus capsid protein VP5* permeabilizes membranes.  |  Denisova, E., et al. 1999. J Virol. 73: 3147-53. PMID: 10074166
2. Antibodies to rotavirus outer capsid glycoprotein VP7 neutralize infectivity by inhibiting virion decapsidation.  |  Ludert, JE., et al. 2002. J Virol. 76: 6643-51. PMID: 12050377
3. Expression of rotavirus capsid protein VP6 in transgenic potato and its oral immunogenicity in mice.  |  Yu, J. and Langridge, W. 2003. Transgenic Res. 12: 163-9. PMID: 12739884
4. Trypsin is associated with the rotavirus capsid and is activated by solubilization of outer capsid proteins.  |  Benureau, Y., et al. 2005. J Gen Virol. 86: 3143-3151. PMID: 16227238
5. Rotavirus anti-VP6 secretory immunoglobulin A contributes to protection via intracellular neutralization but not via immune exclusion.  |  Corthésy, B., et al. 2006. J Virol. 80: 10692-9. PMID: 16956954
6. Assembly of highly infectious rotavirus particles recoated with recombinant outer capsid proteins.  |  Trask, SD. and Dormitzer, PR. 2006. J Virol. 80: 11293-304. PMID: 16971442
7. Intracellular disassembly of infectious rotavirus particles by depletion of Ca2+ sequestered in the endoplasmic reticulum at the end of virus cycle.  |  Ruiz, MC., et al. 2007. Virus Res. 130: 140-50. PMID: 17643542
8. Rotavirus infection induces the phosphorylation of eIF2alpha but prevents the formation of stress granules.  |  Montero, H., et al. 2008. J Virol. 82: 1496-504. PMID: 18032499
9. Geometric mismatches within the concentric layers of rotavirus particles: a potential regulatory switch of viral particle transcription activity.  |  Libersou, S., et al. 2008. J Virol. 82: 2844-52. PMID: 18184711
10. Immunogenicity and virus-like particle formation of rotavirus capsid proteins produced in transgenic plants.  |  Yang, Y., et al. 2011. Sci China Life Sci. 54: 82-9. PMID: 21104033
11. Rotavirus capsid VP6 protein acts as an adjuvant in vivo for norovirus virus-like particles in a combination vaccine.  |  Blazevic, V., et al. 2016. Hum Vaccin Immunother. 12: 740-8. PMID: 26467630
12. Rotavirus capsid VP6 tubular and spherical nanostructures act as local adjuvants when co-delivered with norovirus VLPs.  |  Malm, M., et al. 2017. Clin Exp Immunol. 189: 331-341. PMID: 28407442

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Rotavirus capsid Antibody (2B4) has 11 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Rotavirus capsid Antibody (2B4) Specifications

Alternate Product Name: Rotavirus capsid

Product Clone Name: 2B4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: Rotavirus capsid

Epitope Species:

Product Reactivity/Detection: inner capsid protein VP6

Species Reactivity/Detection: Rotavirus

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF, IHC(P), ELISA

Vialing Information: Each vial contains 50 µg IgG_2b kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for Rotavirus capsid Antibody (2B4): N/A